Sung K L, Kwan M K, Maldonado F, Akeson W H
Department of Orthopaedics, University of California, San Diego, La Jolla 92093-0412.
J Biomech Eng. 1994 Aug;116(3):237-42. doi: 10.1115/1.2895725.
The adhesive interactions of cells with other cells and the extracellular matrix (ECM) play a fundamental role in the organization of cells in differentiated organs, cell motility, and the healing process. The adhesion characteristics of ligament fibroblasts depend on the expression of cell surface molecules and their interaction with the ECM. Although many receptors mediating the effects of ECM components on ligament cell function remain poorly defined, it is known that fibronectin (FN) allows ligament cells to adhere through the VLA-5 receptor (alpha 5 beta 1). A direct measurement of the adhesion between anterior cruciate ligament (ACL) or medial collateral ligament (MCL) fibroblasts and fibronectin matrix proteins was achieved by using a micromanipulation technique to determine the force required to detach an ACL or MCL cell from fibronectin-coated glass. We have found that the adhesion strength is not random, but has well-defined functional relationships with the FN concentration and the seeding time (time allowed for the cell to establish attachment). The adhesion strength (i.e., force required to detach) of ACL cells shows a stronger dependence on FN concentration (1, 2, and 5 micrograms/ml) for short seeding times (15-30 min) than for long seeding times (38-75 min). For MCL cells, the effect of the seeding time on adhesion strength was apparent for all concentrations. For all the seeding times studied and FN concentrations used, MCL cells had higher adhesion strength than ACL cells. The adhesion strengths of ACL and MCL fibroblasts to FN are correlated to cell adhesion area.(ABSTRACT TRUNCATED AT 250 WORDS)
细胞与其他细胞以及细胞外基质（ECM）之间的黏附相互作用在分化器官中细胞的组织、细胞运动以及愈合过程中起着基础性作用。韧带成纤维细胞的黏附特性取决于细胞表面分子的表达及其与细胞外基质的相互作用。尽管许多介导细胞外基质成分对韧带细胞功能影响的受体仍未明确，但已知纤连蛋白（FN）可使韧带细胞通过VLA - 5受体（α5β1）实现黏附。通过使用显微操作技术来确定将前交叉韧带（ACL）或内侧副韧带（MCL）细胞从纤连蛋白包被的玻璃上分离所需的力，从而直接测量ACL或MCL成纤维细胞与纤连蛋白基质蛋白之间的黏附力。我们发现，黏附强度并非随机的，而是与纤连蛋白浓度和接种时间（细胞建立附着所需的时间）存在明确的功能关系。对于短接种时间（15 - 30分钟），ACL细胞的黏附强度（即分离所需的力）对纤连蛋白浓度（1、2和5微克/毫升）的依赖性比对长接种时间（38 - 75分钟）更强。对于MCL细胞，接种时间对黏附强度的影响在所有浓度下都很明显。在所研究的所有接种时间和使用的纤连蛋白浓度下，MCL细胞的黏附强度均高于ACL细胞。ACL和MCL成纤维细胞对纤连蛋白的黏附强度与细胞黏附面积相关。（摘要截断于250字）
