[人嗜T淋巴细胞病毒I型和II型逆转录病毒的分离、测序及超微结构。西班牙静脉注射吸毒者中存在人嗜T淋巴细胞病毒II型b亚型]

[Isolation, sequencing and ultrastructure of HTLV-I and HTLV-II retroviruses. Presence of the HTLV-II subtype b among Spanish intravenous drug addicts].

作者信息

Vallejo A, Casado C, Cantó C, Varela J M, Barge T, Rabella N, Herrera I, García Sáiz A

机构信息

Servicio de Diagnóstico, Centro Nacional de Biología Celular y Retrovirus, Instituto de Salud Carlos III, Majadahonda, Madrid.

出版信息

Med Clin (Barc). 1994 Dec 3;103(19):730-6.

PMID:7799679

Abstract

BACKGROUND

Six cases of HTLV-I/II infection were selected for isolation and characterization of these retrovirus.

METHODS

Detection of anti-HTLV antibodies was carried out by enzyme immunoassay (EIA), immunofluorescence (IFI), and Western blot (WB). Analysis of proviral DNA was performed by PCR. Viral culture and partial sequencing of the pol and pX genes were carried out. Electron microscopy morphologically characterized the viral particles.

RESULTS

Serologic study demonstrated four cases of HTLV-II, one of HTLV-I, and one non-typeable HTLV infections. This last case was confirmed as positive for HTLV-II by PCR. Five new HTLV-II and one HTLV-I infected cell lines have been established by co-culture. Electron microscopy allowed morphologic characterization of the viral particles found in the infected cells. The sequence of the five strains of HTLV-II was identical demonstrating a divergence of 0.49% in the pX region and of 4.5% in the pol region compared with the HTLV-II Mo prototype. Comparison of these sequences with those corresponding to different strains of HTLV-II isolates from American Indians (subtypes b) suggest that these Spanish strains are more closely related with the subtype b than with the subtype a (HTLV-II Mo). Genetic variability study did not reveal any change in the sequence of these stains suggesting that the variability of these retroviruses in very infrequent in the regions studied. The analysis of the pol region of the HTLV-I strain demonstrated a divergence of 3.4% with respect to the sequence of the ATK-1 prototype (Japan) and of 1.7% of the strain HS-35 (Caribbean) showing a greater relation with the Caribbean strains than with those from Japan.

CONCLUSIONS

The presence of HTLV-II subtype has been confirmed among intravenous drug addicts in Spain. Isolation and characterization of the HTLV-I strain demonstrated that this also circulating around Spain despite its South American origin.

摘要

背景

选取6例人嗜T淋巴细胞病毒I/II（HTLV-I/II）感染病例以分离并鉴定这些逆转录病毒。

方法

采用酶免疫测定（EIA）、免疫荧光法（IFI）及蛋白质印迹法（WB）检测抗HTLV抗体。通过聚合酶链反应（PCR）进行前病毒DNA分析。进行病毒培养以及对pol和pX基因进行部分测序。利用电子显微镜对病毒颗粒进行形态学鉴定。

结果

血清学研究显示4例为HTLV-II感染，1例为HTLV-I感染，1例为无法分型的HTLV感染病例。通过PCR证实最后1例病例为HTLV-II阳性。通过共培养建立了5株新的HTLV-II感染细胞系和1株HTLV-I感染细胞系。电子显微镜可对感染细胞中发现的病毒颗粒进行形态学鉴定。5株HTLV-II毒株的序列相同，与HTLV-II Mo原型相比，pX区域的差异为0.49%，pol区域为4.5%。将这些序列与来自美洲印第安人（b亚型）的不同HTLV-II分离株的序列进行比较，结果表明，这些西班牙毒株与b亚型的关系比与a亚型（HTLV-II Mo）更为密切。遗传变异性研究未发现这些毒株序列有任何变化，表明在所研究区域这些逆转录病毒的变异性非常罕见。对HTLV-I毒株pol区域的分析显示，与ATK-1原型（日本）序列的差异为3.4%，与HS-35毒株（加勒比地区）的差异为1.7%，表明与加勒比地区毒株的关系比与日本毒株更为密切。