Bulychev A, Pilon M, Dassen H, van 't Hof R, Vredenberg W, de Kruijff B
Department of Plant Physiology, Wageningen Agricultural University, The Netherlands.
FEBS Lett. 1994 Dec 19;356(2-3):204-6. doi: 10.1016/0014-5793(94)01259-8.
Direct electrical measurements on native chloroplast envelopes reveal that a full-length chloroplast precursor protein causes an increase in the conductivity of the envelope membranes, due to its transit sequence. The conductivity is not influenced by a truncated precursor protein incapable of efficient translocation, suggesting precursor-mediated opening of translocation pores in chloroplast envelopes.
对天然叶绿体被膜进行的直接电学测量表明,由于其转运序列,全长叶绿体前体蛋白会导致被膜膜的电导率增加。电导率不受无法有效转运的截短前体蛋白的影响,这表明前体介导了叶绿体被膜中转运孔的开放。