Hanenberg H, Baumann M, Quentin I, Nagel G, Grosse-Wilde H, von Kleist S, Göbel U, Burdach S, Grunert F
Department of Pediatric Hematology and Oncology, Heinrich Heine-University Medical Center, Düsseldorf, Germany.
Leukemia. 1994 Dec;8(12):2127-33.
The carcinoembryonic antigen (CEA) and the classical non-specific cross-reacting antigens (NCAs) belong to the CEA gene family which is part of the immunoglobulin superfamily. In normal hematopoiesis, CEA gene family members (CGMs) have only been reported on cells of myeloid and monocytic origin. In the present study, we analyzed 62 childhood acute lymphoblastic leukemias (ALLs) and seven surface immunoglobulin positive (sig+) B-cell lines for the expression of the CEA family members CEA, NCA-50/90, NCA-95, NCA-160, CGM1 and CGM7. We demonstrated that members of the CEA family were present in 76% of childhood ALLs of B- and T-cell origin. In ALLs of B-cell origin, 82% of the samples expressed at least one CEA subgroup member: 38% NCA-50/90 (CD66c), 31% NCA-160 (CD66a), and 13% both. Six of seven B-cell lines solely expressed NCA-160. In seven ALL of T-cell origin, sole NCA-160 expression was present in 29% of the cases. CEA and CGM1 were not expressed in childhood ALLs or in the sIg+ B-cell lines. In 15 ALLs and seven B-cell lines which could be analyzed for CGM7 expression, the antigen was not detected. NCA-95 was not expressed in 91% of the B-lineage ALLs, in T-lineage ALLs and in the B-cell lines. However, five B-lineage ALLs showed conflicting data on the binding patterns of two, on leukocytes specifically NCA-95 recognizing antibodies suggesting either expression of unknown forms of NCA-95 or NCA-50/90 or of a yet unknown member of the CEA family in these ALL cells. The expression of CEA subgroup members in childhood ALL cells might have prognostic impacts, as an inverse correlation exists between NCA expression on leukemic blasts and the risk factor white blood count at diagnosis.
癌胚抗原（CEA）和经典的非特异性交叉反应抗原（NCA）属于CEA基因家族，该家族是免疫球蛋白超家族的一部分。在正常造血过程中，CEA基因家族成员（CGM）仅在髓系和单核细胞来源的细胞上有报道。在本研究中，我们分析了62例儿童急性淋巴细胞白血病（ALL）和7个表面免疫球蛋白阳性（sig+）B细胞系中CEA家族成员CEA、NCA-50/90、NCA-95、NCA-160、CGM1和CGM7的表达情况。我们发现，CEA家族成员存在于76%的B细胞和T细胞来源的儿童ALL中。在B细胞来源的ALL中，82%的样本表达至少一种CEA亚组成员：38%为NCA-50/90（CD66c），31%为NCA-160（CD66a），13%同时表达两者。7个B细胞系中有6个仅表达NCA-160。在7例T细胞来源的ALL中，29%的病例仅表达NCA-160。CEA和CGM1在儿童ALL或sIg+ B细胞系中均未表达。在15例ALL和7个可分析CGM7表达的B细胞系中，未检测到该抗原。91%的B系ALL、T系ALL和B细胞系中均未表达NCA-95。然而，5例B系ALL在两种白细胞特异性识别NCA-95的抗体的结合模式上显示出相互矛盾的数据，这表明这些ALL细胞中可能表达未知形式的NCA-95或NCA-50/9（）或CEA家族的一个未知成员。儿童ALL细胞中CEA亚组成员的表达可能具有预后影响，因为白血病原始细胞上NCA的表达与诊断时的危险因素白细胞计数呈负相关。
