Kaul V, Prakash B S
Division of Cattle Physiology, National Dairy Research Institute, Karnal, India.
Trop Anim Health Prod. 1994 Aug;26(3):187-92. doi: 10.1007/BF02241083.
Milk samples collected on days 0, 6, 20, 22 and 24 post insemination from Sahiwal and Tharparkar (zebu) cows, Karan Swiss (Sahiwal x Brown Swiss) and Karan Fries (Tharparkar x Holstein Friesian) crossbred cows and Murrah buffaloes were analysed for progesterone by enzyme-linked immunosorbent assay to determine the accuracy of pregnancy and non pregnancy diagnosis in each of the 3 groups of animals. The accuracy of pregnancy diagnosis was markedly improved in cows when the milk progesterone values on days 20, 22 and 24 were considered together, and a further improvement when the levels on days 0, 6, 20, 22 and 24 were considered together particularly in crossbred cows. In contrast, buffaloes exhibited the highest degree of accuracy from samples collected on day 24, and improvement in accuracy was minimal when values were considered for all 5 days, viz. 0, 6, 20, 22 and 24 post insemination. Overall, the accuracy of positive pregnancy diagnosis was greatest in zebu cows (90.9%) followed by crossbred cows (86.6%) and Murrah buffaloes (76.5%) when milk progesterone values on all 5 days were considered. The accuracy for negative pregnancy diagnosis was 100% on all days. The results suggest that there is a greater variability in oestrous cycle length in cattle than in buffaloes, and embryonic mortality is greater in buffaloes than in cattle.
对在授精后第0天、第6天、第20天、第22天和第24天采集的萨希瓦尔牛和塔尔帕卡尔牛（瘤牛）、卡兰瑞士牛（萨希瓦尔牛×瑞士褐牛）和卡兰弗里西安牛（塔尔帕卡尔牛×荷斯坦弗里西安牛）杂交母牛以及穆拉水牛的牛奶样本进行酶联免疫吸附测定分析，以确定这3组动物中每一组妊娠和未妊娠诊断的准确性。当综合考虑第20天、第22天和第24天的牛奶孕酮值时，母牛妊娠诊断的准确性显著提高；当综合考虑第0天、第6天、第20天、第22天和第24天的水平时，准确性进一步提高，尤其是在杂交母牛中。相比之下，水牛在第24天采集的样本中表现出最高的准确性，当考虑授精后所有5天（即第0天、第6天、第20天、第22天和第24天）的值时，准确性的提高最小。总体而言，当综合考虑所有5天的牛奶孕酮值时，瘤牛母牛的阳性妊娠诊断准确性最高（90.9%），其次是杂交母牛（86.6%）和穆拉水牛（76.5%）。所有天数的阴性妊娠诊断准确性均为100%。结果表明，牛的发情周期长度变异性比水牛大，水牛的胚胎死亡率比牛高。
