Kageyama S, Tsomides T J, Sykulev Y, Eisen H N
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139.
J Immunol. 1995 Jan 15;154(2):567-76.
We determined equilibrium constants for the binding of 16 peptides (based on four T cell epitopes) to three MHC class I proteins (A2, Kb, and Ld) on intact cells and estimated the number of accessible peptide-binding sites on these cells. From these results, and the concentrations of peptides required to sensitize target cells for lysis by CD8+ CTL, we conclude that the critical number of peptide-MHC complexes required per target cell for the activation of CTL responses varies with different combinations of peptide-MHC complexes and CTL clones from several thousand complexes to fewer than ten per target cell.
我们测定了16种肽（基于4个T细胞表位）与完整细胞上三种I类主要组织相容性复合体蛋白（A2、Kb和Ld）结合的平衡常数，并估算了这些细胞上可及的肽结合位点数量。根据这些结果以及使靶细胞对CD8⁺细胞毒性T淋巴细胞（CTL）介导的裂解敏感所需的肽浓度，我们得出结论：激活CTL反应所需的每个靶细胞的关键肽 - 主要组织相容性复合体复合物数量，随肽 - 主要组织相容性复合体复合物和CTL克隆的不同组合而变化，从数千个复合物到每个靶细胞少于十个。
