SV40微型染色体的再复制在链延伸阶段受到抑制。

Krude T, Winter A, Knippers R

Fakultät für Biologie, Universität Konstanz, Germany.

Nucleic Acids Res. 1994 Dec 11;22(24):5265-70. doi: 10.1093/nar/22.24.5265.

The template activities of protein-free SV40 DNA and SV40 minichromosomes for DNA re-replication are compared in in vitro replication assays. Density substitution experiments and two-dimensional gel electrophoresis show that protein-free DNA can replicate for at least two cycles whereas salt-treated minichromosomes replicate only once. Re-replication of minichromosomes is blocked at the stage of replicative chain elongation suggesting that replicatively assembled chromatin has structural features that prevent a second round of replication.

在体外复制试验中，对无蛋白质的SV40 DNA和SV40微型染色体用于DNA再复制的模板活性进行了比较。密度置换实验和二维凝胶电泳表明，无蛋白质的DNA可以复制至少两个周期，而经盐处理的微型染色体只能复制一次。微型染色体的再复制在复制链延伸阶段受阻，这表明复制组装的染色质具有阻止第二轮复制的结构特征。