非洲爪蟾脊髓的中枢神经系统髓磷脂和少突胶质细胞——而非视神经的——对轴突生长不具有容许性。
CNS myelin and oligodendrocytes of the Xenopus spinal cord--but not optic nerve--are nonpermissive for axon growth.
作者信息
Lang D M, Rubin B P, Schwab M E, Stuermer C A
机构信息
University of Konstanz, Faculty of Biology, Germany.
出版信息
J Neurosci. 1995 Jan;15(1 Pt 1):99-109. doi: 10.1523/JNEUROSCI.15-01-00099.1995.
In vitro assays reveal that myelin and oligodendrocytes of the Xenopus spinal cord (SC) are--unlike corresponding components of the optic nerve/tectum (OT)--nonpermissive substrates for regenerating retinal axons. The number of growth cones that crossed SC oligodendrocytes is low but increases significantly (four- to fivefold) in the presence of the antibody IN-1, in which case their numbers are similar to the number of growth cones (approximately 60%) that cross OT oligodendrocytes with or without IN-1. IN-1 neutralizes neurite growth inhibitors (NI) of rat CNS myelin, indicating that mammalian-like NI are associated with Xenopus SC myelin and oligodendrocytes but not with the OT. IN-1 immunocytochemistry on sections supports this view: SC myelin was stained with IN-1, whereas OT myelin and PNS myelin were not.
体外实验表明，非洲爪蟾脊髓（SC）的髓磷脂和少突胶质细胞——与视神经/视顶盖（OT）的相应成分不同——是视网膜轴突再生的非许可性底物。穿过SC少突胶质细胞的生长锥数量较少，但在抗体IN-1存在的情况下显著增加（四到五倍），在这种情况下，它们的数量与穿过有或没有IN-1的OT少突胶质细胞的生长锥数量相似（约60%）。IN-1可中和大鼠中枢神经系统髓磷脂的神经突生长抑制剂（NI），这表明类似哺乳动物的NI与非洲爪蟾SC髓磷脂和少突胶质细胞相关，但与OT无关。切片上的IN-1免疫细胞化学支持这一观点：SC髓磷脂被IN-1染色，而OT髓磷脂和周围神经系统髓磷脂未被染色。
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