通过放射自显影法测量大鼠脑中ω-芋螺毒素MVIICnle结合位点的分布。

The distribution of omega-conotoxin MVIICnle-binding sites in rat brain measured by autoradiography.

作者信息

Filloux F, Karras J, Imperial J S, Gray W R, Olivera B M

机构信息

Department of Neurology, University of Utah, Salt Lake City 84132.

出版信息

Neurosci Lett. 1994 Sep 12;178(2):263-6. doi: 10.1016/0304-3940(94)90774-9.

DOI:10.1016/0304-3940(94)90774-9

PMID:7824207

Abstract

An analogue of omega-conotoxin MVIIC, [125I]omega-MVIICnle, has been employed in an autoradiographic assay to define the distribution of binding sites in rat brain of this neuronal calcium channel antagonist. In comparison with N-type channels (labeled by [125I]omega conotoxin GVIA), omega-MVIICnle sites are much denser in cerebellum (molecular layer) than in forebrain. Binding in thalamus is also comparatively high for omega-MVIICnle. Under these conditions, [125I]omega-MVIICnle binding to rat brain sections is not displaceable by the N-channel antagonist, omega-conotoxin GVIA. The calcium channel blocker [125I]omega-conotoxin MVIICnle labels a unique set of binding sites in mammalian brain.

摘要

ω-芋螺毒素MVIIC的类似物[125I]ω-MVIICnle已被用于放射自显影分析，以确定这种神经元钙通道拮抗剂在大鼠脑中结合位点的分布。与N型通道（由[125I]ω-芋螺毒素GVIA标记）相比，ω-MVIICnle位点在小脑（分子层）中的密度比在前脑中高得多。ω-MVIICnle在丘脑中的结合也相对较高。在这些条件下，[125I]ω-MVIICnle与大鼠脑切片的结合不能被N通道拮抗剂ω-芋螺毒素GVIA取代。钙通道阻滞剂[125I]ω-芋螺毒素MVIICnle标记了哺乳动物脑中一组独特的结合位点。