鼠伤寒沙门氏菌温度敏感型核糖核酸聚合酶突变体，其β'亚基存在缺陷。
Temperature-sensitive ribonucleic acid polymerase mutant of Salmonella typhimurium with a defect in the beta' subunit.
作者信息
Young B S, Guterman S K, Wright A
出版信息
J Bacteriol. 1976 Sep;127(3):1292-7. doi: 10.1128/jb.127.3.1292-1297.1976.
Abstract
Localized mutagenes of Salmonella typhimurium followed by a [3H]uridine enrichment procedure yielded a temperature-sensitive strain with a mutation in the rpo region of the chromosome. Ribonucleic acid (RNA) polymerase (EC 2.7.7.6; nucleoside triphosphate: RNA nucleotidyltransferase) purified from this mutant was considerably less active at the nonpermissive temperature than wild-type enzyme. Furthermore, the enzyme from this mutant, unlike RNA polymerase of previously isolated temperature-sensitive mutants, was as thermostable as wild-type enzyme when preincubated at 50 degrees C. Subunit reconstitution experiments have shown that the temperature sensitivity is caused by an alteration in the beta' subunit of the enzyme.
摘要
对鼠伤寒沙门氏菌进行局部诱变，随后采用[3H]尿苷富集程序，得到了一株在染色体rpo区域发生突变的温度敏感型菌株。从该突变体中纯化的核糖核酸（RNA）聚合酶（EC 2.7.7.6；核苷三磷酸：RNA核苷酸转移酶）在非允许温度下的活性比野生型酶低得多。此外，与先前分离的温度敏感型突变体的RNA聚合酶不同，该突变体的酶在50℃预孵育时与野生型酶一样耐热。亚基重组实验表明，温度敏感性是由该酶β'亚基的改变引起的。
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