Rao J K, Ramesh D V, Rao K P
Biomaterials Laboratory, Central Leather Research Institute, Adyar, Madras, India.
J Biomater Sci Polym Ed. 1994;6(5):391-8. doi: 10.1163/156856294x00383.
The preparation and characterization of biodegradable gelatin microspheres for the controlled release of peptides and proteins has been investigated. Bovine serum albumin (BSA) was chosen for incorporation into the gelatin microspheres and the spheres were characterized for the in vitro release of BSA and other properties. BSA was labelled with fluorescein isothiocyanate (FITC) for easy analysis. FITC-BSA was entrapped into the gelatin microspheres using a polymer dispersion technique developed in our earlier studies. The morphological characteristics of microspheres were analysed by optical and scanning electron microscopy (SEM). The optical and SEM photographs of FITC-BSA microspheres showed the solid spherical nature of the spheres. The entrapment efficiency of FITC-BSA was about 62%. The in vitro release pattern of FITC-BSA showed that 51% of the entrapped drug was released during the first day and the release followed approximate zero order kinetics from day 2 onwards. The total release of FITC-BSA lasted for about 8 days. SDS-PAGE analysis revealed that BSA was not degraded by this preparation of microspheres.
对用于肽和蛋白质控释的可生物降解明胶微球的制备及特性进行了研究。选择牛血清白蛋白（BSA）掺入明胶微球中，并对微球进行了BSA体外释放及其他特性的表征。用异硫氰酸荧光素（FITC）标记BSA以便于分析。采用我们早期研究中开发的聚合物分散技术将FITC-BSA包封到明胶微球中。通过光学显微镜和扫描电子显微镜（SEM）分析微球的形态特征。FITC-BSA微球的光学照片和SEM照片显示微球呈实心球形。FITC-BSA的包封率约为62%。FITC-BSA的体外释放模式表明，在第一天有51%的包封药物被释放，从第二天起释放遵循近似零级动力学。FITC-BSA的总释放持续约8天。SDS-PAGE分析表明，该微球制剂不会使BSA降解。
