维甲酸对大鼠气管外植体中粘蛋白mRNA的调节：对放线菌素D、环己酰亚胺、信号转导效应物及反义寡脱氧核苷酸的反应

Retinoic acid modulation of mucin mRNA in rat tracheal explants: response to actinomycin D, cycloheximide, signal transduction effectors and antisense oligodeoxynucleotide.

作者信息

Bhattacharyya S N, Ashbaugh P, Kaufman B, Manna B

机构信息

Department of Clinical Investigation, William Beaumont Army Medical Center, El Paso, Texas 79920.

出版信息

Inflammation. 1994 Dec;18(6):565-74. doi: 10.1007/BF01535255.

DOI:10.1007/BF01535255

Abstract

Factors affecting the retinoic acid modulated expression of mucin mRNA in rat tracheal cultures were studied. Actinomycin D had no effect on mucin mRNA in cultures grown with retinoic acid (RA+). The usual precipitous drop in mucin mRNA in cultures lacking retinoic acid (RA-) was prevented by actinomycin D. Cycloheximide also had no effect on mucin mRNA in RA+ cultures, but, like actinomycin D, it prevented the precipitous drop in mucin mRNA in RA- cultures. cAMP agonists had some marginal effects on the mucin mRNA, but none as dramatic as those noted by actinomycin D and cycloheximide in the RA- cultures. An antisense oligomer (18 bases) to rat mucin cDNA inhibited the mucin mRNA expression in RA+ cultures.

摘要

研究了影响维甲酸调节大鼠气管培养物中粘蛋白mRNA表达的因素。放线菌素D对用维甲酸培养的细胞（RA+）中的粘蛋白mRNA没有影响。在缺乏维甲酸的培养物（RA-）中，粘蛋白mRNA通常的急剧下降被放线菌素D阻止。环己酰亚胺对RA+培养物中的粘蛋白mRNA也没有影响，但是，与放线菌素D一样，它阻止了RA-培养物中粘蛋白mRNA的急剧下降。环磷酸腺苷激动剂对粘蛋白mRNA有一些轻微影响，但没有放线菌素D和环己酰亚胺在RA-培养物中所观察到的那么显著。针对大鼠粘蛋白cDNA的反义寡聚体（18个碱基）抑制了RA+培养物中的粘蛋白mRNA表达。