从人中性粒细胞中纯化溶菌酶，并开发用于细胞和血浆定量的酶联免疫吸附测定法。
Purification of lysozyme from human neutrophils, and development of an ELISA for quantification in cells and plasma.
作者信息
Lollike K, Kjeldsen L, Sengeløv H, Borregaard N
机构信息
Department of Hematology, National University Hospital, Rigshopitalet, Copenhagen, Denmark.
出版信息
Leukemia. 1995 Jan;9(1):206-9.
Lysozyme was purified from exocytosed granule material from PMA-stimulated human neutrophils by polyethyleneglycol precipitation, cation exchange chromatography and molecular sieve chromatography. Rabbit antibodies were biotinylated and affinity purified on a lysozyme column, for subsequent development of a novel ELISA. This ELISA for lysozyme is sensitive and accurate, and applicable to determination of lysozyme in neutrophils and plasma.
通过聚乙二醇沉淀、阳离子交换色谱法和分子筛色谱法从经佛波酯（PMA）刺激的人中性粒细胞的胞吐颗粒物质中纯化溶菌酶。将兔抗体进行生物素化，并在溶菌酶柱上进行亲和纯化，以用于后续新型酶联免疫吸附测定（ELISA）的开发。这种溶菌酶ELISA灵敏且准确，适用于测定中性粒细胞和血浆中的溶菌酶。
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