Bailly M, Zebda N, Dore J F
INSERM U218, Centre Léon Bérard, Lyon, France.
Anticancer Res. 1994 Sep-Oct;14(5A):1791-9.
We previously established a model to study human tumor spontaneous metastasis in immunosuppressed newborn rats. Different observations suggested that the development of pulmonary metastases of human melanoma cells in this animal might reflect, at least partially, an organ specificity phenomenon. We thus examined the "soil" property of the rat lung in terms of growth stimulating activity and specific interactions between tumor cells and pulmonary cells. We could not demonstrate any melanoma cell growth-promoting activity in lung conditioned medium. On the other hand, tumor cell adhesion was drastically enhanced when measured on pulmonary fibroblast monolayers or derived extracellular matrix. Adsorption of lysates of 7GP122 highly metastatic melanoma variant on viable total lung cells enabled us to detect at least 10 proteins or protein subunits which could specifically interact with pulmonary cell membranes, while only one of these proteins was detectable when the same experiment was performed with control liver cells. Conversely, we could show that there exist several corresponding structures on pulmonary cells which could adsorb on tumor cells. Thus, specific cell surface adhesion molecules, leading to specific adhesion between melanoma cells and pulmonary cells, may lead to preferential metastatic development in rat lung.
我们之前建立了一个模型来研究免疫抑制新生大鼠体内的人类肿瘤自发转移。不同的观察结果表明，人类黑色素瘤细胞在这种动物体内肺转移的发生可能至少部分反映了一种器官特异性现象。因此，我们从生长刺激活性以及肿瘤细胞与肺细胞之间的特异性相互作用方面研究了大鼠肺的“土壤”特性。我们未能在肺条件培养基中证明任何黑色素瘤细胞生长促进活性。另一方面，当在肺成纤维细胞单层或其衍生的细胞外基质上进行测量时，肿瘤细胞的黏附显著增强。7GP122高转移性黑色素瘤变体的裂解物吸附在存活的全肺细胞上，使我们能够检测到至少10种可与肺细胞膜特异性相互作用的蛋白质或蛋白质亚基，而在用对照肝细胞进行相同实验时，这些蛋白质中只有一种可被检测到。相反，我们可以证明肺细胞上存在几种可吸附在肿瘤细胞上的相应结构。因此，导致黑色素瘤细胞与肺细胞之间特异性黏附的特定细胞表面黏附分子，可能导致大鼠肺中优先发生转移。
