杀草强的致突变性与毒性。III. 微生物试验。

Mutagenicity and toxicity of amitrole. III. Microbial tests.

作者信息

Bamford D, Sorsa M, Gripenberg U, Laamanen I, Meretoja T

出版信息

Mutat Res. 1976 Jul;40(3):197-202. doi: 10.1016/0165-1218(76)90045-8.

Abstract

Amitrole (3-amino-1,2,4-triazole) inhibits bacterial growth both in Escherichia coli and Salmonella typhimurium at a concentration of 0.5% in minimal medium. Repression of growth already occurs at a concentration of 0.1% of amitrole in this medium. In complete medium the bacteria tolerate concentrations of amitrole as high as 1.7-2.4% before growth ceases. Mutagenicity was tested by differential growth comparisons on E. coli strains W 3110 thy pol A1, defective in DNA polymerase I, and its revertant pol A+. Known mutagens (MMS, NTG, mitomycin C) were used as positive controls. Analogous negative results were also obtained in a revertant test when several trp mutant strains of Salmonella were used.

摘要

灭草隆（3-氨基-1,2,4-三唑）在基本培养基中浓度为0.5%时可抑制大肠杆菌和鼠伤寒沙门氏菌的生长。在此培养基中，灭草隆浓度为0.1%时就已出现生长抑制。在完全培养基中，细菌在生长停止前可耐受高达1.7 - 2.4%的灭草隆浓度。通过对DNA聚合酶I缺陷的大肠杆菌菌株W 3110 thy pol A1及其回复株pol A+进行差异生长比较来测试致突变性。已知诱变剂（甲基磺酸甲酯、亚硝基胍、丝裂霉素C）用作阳性对照。当使用沙门氏菌的几种色氨酸突变菌株进行回复试验时，也得到了类似的阴性结果。