从圣保罗儿童粪便中分离出的产肠毒素大肠杆菌中定植因子抗原（CFAs）的流行情况及对HeLa细胞的黏附性

Prevalence of colonization factor antigens (CFAs) and adherence to HeLa cells in enterotoxigenic Escherichia coli isolated from feces of children in São Paulo.

作者信息

Guth B E, Aguiar E G, Griffin P M, Ramos S R, Gomes T A

机构信息

Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, São Paulo, Brazil.

出版信息

Microbiol Immunol. 1994;38(9):695-701. doi: 10.1111/j.1348-0421.1994.tb01844.x.

DOI:10.1111/j.1348-0421.1994.tb01844.x

PMID:7854210

Abstract

Fifty-eight enterotoxigenic Escherichia coli (ETEC) strains, isolated from children with and without diarrhea in São Paulo, were examined for the presence of colonization factor antigens (CFAs) and their ability to adhere to HeLa cells. Antisera to CFA/I, the coli surface (CS) antigens CS1CS3, CS2CS3, and CS2 of CFA/II, CFA/III, and CS5CS6 and CS6 of CFA/IV were used. CFAs were identified in 43% of the ETEC strains: 40% of the CFAs strains with CFAs harbored CFA/I, 24% carried CFA/II (CS1CS3), 24% carried CFA/IV (CS6), and 12% carried CFA/IV (CS5CS6). CFAs occurred mainly among ETEC strains producing only heat-stable (ST-I) enterotoxin and in strains also producing heat-labile toxin (LT-I). No ETEC strains tested expressed CFA/III. A marked change in serotypes of ST-I-producing strains was found in São Paulo between 1979 and 1990. Adherence to HeLa cells was detected in 14% of the ETEC strains. All of them had a diffuse adherence pattern and produced only ST-I, and 88% carried CS6 antigen.

摘要

从圣保罗腹泻和未腹泻儿童中分离出58株产肠毒素大肠杆菌（ETEC），检测其定居因子抗原（CFA）的存在情况及其黏附HeLa细胞的能力。使用了针对CFA/I、CFA/II的菌毛表面（CS）抗原CS1CS3、CS2CS3和CS2、CFA/III以及CFA/IV的CS5CS6和CS6的抗血清。在43%的ETEC菌株中鉴定出CFA：携带CFA的菌株中，40%含有CFA/I，24%携带CFA/II（CS1CS3），24%携带CFA/IV（CS6），12%携带CFA/IV（CS5CS6）。CFA主要出现在仅产生热稳定（ST-I）肠毒素的ETEC菌株以及也产生不耐热毒素（LT-I）的菌株中。所检测的ETEC菌株均未表达CFA/III。1979年至1990年间，圣保罗产ST-I菌株的血清型发生了显著变化。在14%的ETEC菌株中检测到对HeLa细胞的黏附。它们均具有弥漫性黏附模式，仅产生ST-I，且88%携带CS6抗原。