Bonaz B, Plourde V, Taché Y
CURE/UCLA Digestive Disease Center, VA Wadsworth Medical Center, Department of Medicine 90073.
J Comp Neurol. 1994 Nov 8;349(2):212-22. doi: 10.1002/cne.903490205.
Previous neuropharmacological studies indicate that brain peptides are involved in mediating gastric stasis induced by abdominal surgery. Central pathways activated by abdominal surgery were investigated in the rat by using Fos protein as a marker of neuronal activation. Abdominal surgery (laparotomy alone or combined with cecal manipulation) was performed under brief enflurane anesthesia (7-8 minutes), and 1 hour later rats were killed and brains processed for Fos immunoreactivity. Double labeling with Fos and arginine vasopressin, oxytocin, or tyrosine hydroxylase antibodies was also performed. Abdominal surgery induced Fos staining in the nucleus tractus solitarii, paraventricular and supraoptic nuclei of the hypothalamus, locus coeruleus, and ventrolateral medulla. After abdominal surgery, 18-25% of vasopressin and 18-33% of oxytocin-labeled cells were found to be Fos positive in the paraventricular nucleus and 15% of activated cells in the nucleus tractus solitarii were positive for tyrosine hydroxylase immunoreactivity. Enflurane alone induced c-fos expression in the same brain area; however, the number of Fos-positive cells and double-labeled cells were decreased two- to fivefold and three- to eightfold, respectively, compared with the abdominal surgery groups. These data show that abdominal surgery induced activation of specific hypothalamic, pontine, and medullary neurons. These findings may have implications for the understanding of central mechanisms involved in mediating gastric ileus following abdominal surgery.
以往的神经药理学研究表明，脑肽参与介导腹部手术引起的胃淤滞。通过使用Fos蛋白作为神经元激活的标志物，在大鼠中研究了腹部手术激活的中枢通路。在短暂的恩氟烷麻醉（7 - 8分钟）下进行腹部手术（单独开腹或联合盲肠操作），1小时后处死大鼠，对大脑进行Fos免疫反应性处理。还进行了Fos与精氨酸加压素、催产素或酪氨酸羟化酶抗体的双重标记。腹部手术诱导了孤束核、下丘脑室旁核和视上核、蓝斑和延髓腹外侧的Fos染色。腹部手术后，在室旁核中发现18 - 25%的加压素标记细胞和18 - 33%的催产素标记细胞为Fos阳性，孤束核中15%的激活细胞为酪氨酸羟化酶免疫反应阳性。单独使用恩氟烷在相同脑区诱导c - fos表达；然而，与腹部手术组相比，Fos阳性细胞和双重标记细胞的数量分别减少了2至5倍和3至8倍。这些数据表明腹部手术诱导了特定下丘脑、脑桥和延髓神经元的激活。这些发现可能对理解腹部手术后介导胃麻痹的中枢机制具有重要意义。
