Jiang S, Ulrich C
Department of Internal Medicine, University of Cincinnati College of Medicine, OH 45267-0595.
Biochem Biophys Res Commun. 1995 Feb 27;207(3):883-90. doi: 10.1006/bbrc.1995.1268.
Through binding to high affinity receptors, secretin is the major hormonal stimulant of ductular bicarbonate secretion in the pancreas, contributes toward enzyme secretion from pancreatic acinar cells, and has been implicated as a pancreatic growth factor. In this work, we utilized hybridization screening of a human pancreatic cDNA library to clone a 1.8-kb cDNA encoding a 440 amino acid protein 81% identical to the rat pancreatic secretin receptor. COS-7 cells transfected with this cDNA bound secretin with a high affinity, and vasoactive intestinal peptide with a lower affinity, appropriate for a secretin receptor. Receptor-bearing COS-7 cells also exhibited an increase in cyclic AMP generation in response to secretin stimulation. Studies analyzing the role of this receptor in human pancreatic physiology and pathophysiology are currently underway in our laboratory.
通过与高亲和力受体结合，促胰液素是胰腺导管碳酸氢盐分泌的主要激素刺激物，有助于胰腺腺泡细胞分泌酶，并且被认为是一种胰腺生长因子。在这项研究中，我们利用对人胰腺cDNA文库进行杂交筛选，克隆出一个1.8 kb的cDNA，其编码一种440个氨基酸的蛋白质，与大鼠胰腺促胰液素受体有81%的同一性。用该cDNA转染的COS-7细胞以高亲和力结合促胰液素，以较低亲和力结合血管活性肠肽，这符合促胰液素受体的特征。表达该受体的COS-7细胞在促胰液素刺激下也表现出环磷酸腺苷生成增加。目前我们实验室正在进行分析该受体在人类胰腺生理和病理生理中作用的研究。
