Okada Y, Petersen C C, Kubo M, Morishima S, Tominaga M
Department of Cellular and Molecular Physiology, National Institute for Physiological Sciences, Okazaki, Japan.
Jpn J Physiol. 1994;44(4):403-9. doi: 10.2170/jjphysiol.44.403.
During osmotic swelling of a human intestinal epithelial cell line, stepwise closing unitary events of Cl- channels could be observed in cell-attached patches upon application of large positive potentials only when inactivating whole-cell Cl- currents were simultaneously observed. The closing process became faster with increasing positive command pulses. The unitary conductance was around 32 pS at 60 mV, 37 pS at +100 mV, and 47 pS at +140 mV exhibiting outward rectification.
在人肠上皮细胞系的渗透肿胀过程中，仅当同时观察到全细胞Cl⁻电流失活时，在细胞贴附式膜片上施加较大正电位时，可观察到Cl⁻通道逐步关闭的单一事件。随着正指令脉冲增加，关闭过程加快。在60 mV时单通道电导约为32 pS，在 +100 mV时为37 pS，在 +140 mV时为47 pS，呈现外向整流。
