Finnen R L, Rochon D M
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.
Virology. 1995 Feb 20;207(1):282-6. doi: 10.1006/viro.1995.1078.
Coinfections of synthetic transcripts from a cDNA clone of cucumber necrosis virus (CNV) and cDNA clones of defective interfering (DI) RNAs were previously demonstrated to contain DI-like RNAs approximately twice the size of the DI RNA used for co-inoculation. Analysis of these RNAs revealed that they are head-to-tail repeats of CNV DI RNA sequence (dimers). Sequence analysis of 21 cloned dimer junctions indicated that approximately half of the junction sequences correspond to precise fusions of monomer units. A cDNA clone corresponding to a dimer of DI RNA 42 was constructed. Synthetic DI RNA 42 dimer transcripts were biologically active in coinfections, resulting in the accumulation of high levels of DI RNA 42 monomers. The possibility that dimers serve as templates for the generation of DI RNA monomers is discussed.
先前已证明，黄瓜坏死病毒（CNV）cDNA克隆的合成转录本与缺陷干扰（DI）RNA的cDNA克隆共感染时，所产生的类似DI的RNA大小约为用于共接种的DI RNA的两倍。对这些RNA的分析表明，它们是CNV DI RNA序列的头尾重复序列（二聚体）。对21个克隆的二聚体连接点进行序列分析表明，大约一半的连接点序列对应于单体单元的精确融合。构建了一个与DI RNA 42二聚体相对应的cDNA克隆。合成的DI RNA 42二聚体转录本在共感染中具有生物活性，导致高水平的DI RNA 42单体积累。文中讨论了二聚体作为DI RNA单体生成模板的可能性。
