Dror Y, Stern F, Komarnitsky M
Department of Biochemistry, Food Sciences and Nutrition, Faculty of Agriculture-Rehovot, Hebrew University of Jerusalem, Israel.
Int J Vitam Nutr Res. 1994;64(4):257-62.
To measure the activation coefficient (AC) of erythrocyte glutathione reductase (GR), suspended whole blood was lysed in a preincubation solution, with or without 100 microM flavin adenine dinucleotide (FAD). Upon addition of the reaction mixture, FAD concentration decreased about 10-fold. No AC values < 1 were obtained in any of the subjects. The range of unstimulated activity per g hemoglobin (Hb) was 5 to 12 U. AC values of healthy subjects (1.3) decreased to about 1.15 after vitamin supplementation of 1 RDA for 3 wk. In healthy young subjects consumption of dietary riboflavin at levels as low as 0.5 mg/d resulted in an AC of 1.6.
为测定红细胞谷胱甘肽还原酶（GR）的激活系数（AC），将悬浮的全血在预孵育溶液中裂解，该溶液添加或不添加100微摩尔黄素腺嘌呤二核苷酸（FAD）。加入反应混合物后，FAD浓度降低了约10倍。在任何受试者中均未获得<1的AC值。每克血红蛋白（Hb）的未刺激活性范围为5至12 U。健康受试者的AC值（1.3）在补充1个推荐膳食摄入量（RDA）的维生素3周后降至约1.15。在健康的年轻受试者中，每天摄入低至0.5毫克的膳食核黄素会导致AC为1.6。
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