Navascués J, Moujahid A, Quesada A, Cuadros M A
Departamento de Biología Celular, Facultad de Ciencias, Universidad de Granada, Spain.
J Comp Neurol. 1994 Dec 8;350(2):171-86. doi: 10.1002/cne.903500203.
Immunocytochemical techniques were used in conjunction with the QH1 antibody to study the morphological characteristics and distribution of microglia in the avascular retina of an avian species (the quail). The majority of microglial cells appeared in the outer and inner plexiform layers throughout the entire retina, whereas a few microglial cells in the nerve fiber layer were seen only in the central zone of the retina, near the optic nerve head. In the outer plexiform layer, microglial cells were star-shaped, with processes that ramified profusely in the horizontal plane. Fine process tips extended outward radially, insinuating themselves among the photoreceptors. A regular mosaic-like arrangement of microglial cells was evident in the outer plexiform layer, with no overlapping between adjacent cell territories. Microglial cells in the inner plexiform layer ramified through the entire width of this layer, showing radial and horizontal processes. Microglia in the inner plexiform layer also tended to be regularly distributed in a mosaic-like fashion, although there was slight overlapping between adjacent cell territories. Microglia density in this layer was approximately twice that in the outer plexiform layer. This pattern of microglial distribution was similar to that described in vascular retinae of several species of mammals, a finding that suggest that blood vessels are not responsible for the final locations of microglia in the adult retina, and that microglial precursors must migrate through long distances before they reach their precise destination.
免疫细胞化学技术与QH1抗体联合使用，以研究一种鸟类（鹌鹑）无血管视网膜中小胶质细胞的形态特征和分布。大多数小胶质细胞出现在整个视网膜的外网状层和内网状层，而神经纤维层中的少数小胶质细胞仅见于视网膜中央区，靠近视神经乳头。在外网状层中，小胶质细胞呈星形，其突起在水平面上大量分支。细小的突起尖端径向向外延伸，插入光感受器之间。在外网状层中，小胶质细胞呈现出规则的镶嵌状排列，相邻细胞区域之间没有重叠。内网状层中的小胶质细胞在该层的整个宽度上分支，显示出径向和水平的突起。内网状层中的小胶质细胞也倾向于以镶嵌状方式规则分布，尽管相邻细胞区域之间存在轻微重叠。该层中小胶质细胞的密度约为外网状层的两倍。这种小胶质细胞分布模式与几种哺乳动物血管视网膜中描述的模式相似，这一发现表明血管并非成年视网膜中小胶质细胞最终定位的原因，并且小胶质细胞前体在到达其精确目的地之前必须迁移很长距离。
