Heiss W D, Wienhard K, Graf R, Löttgen J, Pietrzyk U, Wagner R
Max-Planck-Institut für Neurologische Forschung, Neurologische Universitätsklinik, Köln, Germany.
J Nucl Med. 1995 Mar;36(3):493-8.
A commercial high-resolution scanner designed for clinical PET studies was tested for its applicability to investigate cerebral metabolism and blood flow in cats.
Cerebral blood flow, CMRO2, CBV and CMRglc were determined repeatedly using 15O steady-state oxygen methods and 18F-fluorodeoxyglucose (FDG). Metabolic and blood flow images of 14 contiguous 3-mm PET slices were compared to histological sections in four control animals. In another six cats, hemodynamic and metabolic changes were followed by serial multi-tracer PET for 24 hr after permanent occlusion of the left middle cerebral artery (MCA). Pattern and extent of changes of the physiological variables were related to the final infarct verified in matched histological sections.
At spatial resolutions (FWHM) of 3.6 mm in transaxial planes and 4.0 mm axially, details of the gross anatomy of the cat brain were distinguished best in the FDG images. Cerebral blood flow, CMRO2 and CMRglc values measured in the cortex, white matter and basal ganglia were in the range of common autoradiographic results. Immediately after MCA occlusion, there was widespread decrease in blood flow, but metabolism was preserved at values, which suggest viable tissue. With time, the areas of increased oxygen extraction fraction (OEF) moved from the center to the periphery of the MCA territory.
High-resolution PET can be used for repeat, quantitative imaging of blood flow and metabolism in small animals such as the cat. After MCA occlusion, the changes in blood flow and metabolism can be followed over time and can be related to the final morphological lesion.
对一台专为临床正电子发射断层扫描（PET）研究设计的商用高分辨率扫描仪进行了测试，以评估其在研究猫脑代谢和血流方面的适用性。
使用15O稳态氧方法和18F-氟脱氧葡萄糖（FDG）反复测定脑血流量、氧代谢率（CMRO2）、脑血容量（CBV）和葡萄糖代谢率（CMRglc）。将14个连续的3毫米PET切片的代谢和血流图像与4只对照动物的组织学切片进行比较。在另外6只猫中，在永久性闭塞左大脑中动脉（MCA）后，通过连续多示踪剂PET跟踪血流动力学和代谢变化24小时。生理变量变化的模式和范围与匹配组织学切片中证实的最终梗死灶相关。
在横断面平面上空间分辨率（半高宽）为3.6毫米、轴向分辨率为4.0毫米时，猫脑大体解剖结构的细节在FDG图像中分辨得最好。在皮质、白质和基底神经节中测得的脑血流量、CMRO2和CMRglc值在常见放射自显影结果范围内。MCA闭塞后立即出现广泛的血流减少，但代谢保持在提示组织存活的值。随着时间的推移，氧摄取分数（OEF）增加的区域从MCA区域的中心移向周边。
高分辨率PET可用于对猫等小动物的血流和代谢进行重复、定量成像。MCA闭塞后，可随时间跟踪血流和代谢变化，并可将其与最终的形态学病变相关联。
