Schweizer M, Neumann-Haefelin D
Abteilung Virologie, University of Frelburg, Germany.
Virology. 1995 Mar 10;207(2):577-82. doi: 10.1006/viro.1995.1120.
The relationship between primate foamy viruses was determined by comparing a 425-bp DNA segment obtained by PCR using primers homologous to highly conserved portions of the pol gene. The phylogenetic tree of 14 foamy viruses investigated reflects the relationship between their host species: A cluster of Asian Old World monkey foamy viruses including simian foamy virus (SFV) prototypes 1 and 2 (isolated from Macaca cyclopsis) is separated from African Old World foamy viruses including prototype SFV-3 and SFV-3 strain LK-3 (isolated from African green monkeys, Cercopithecus aethiops). These two clusters of Old World monkey foamy viruses are more distantly related to a cluster of ape and human foamy viruses including prototypes SFV-6, SFV-7, SFV cpz (all isolated from chimpanzees), and human foamy virus (HFV). The New World prototype SFV-8 (isolated from a spider monkey, Ateles sp.) is distinct from the Old World cluster. Our own foamy virus isolates from a rhesus monkey (Macaca mulatta) and an African green monkey were grouped to the Asian or the African Old World monkey foamy virus cluster, respectively. The foamy virus sequences obtained from lymphocytes of two humans, one exposed to African green monkeys and the other to cultured HFV, were compared. The first sequence was closely related to the African Old World monkey foamy virus cluster, whereas the second was identical to HFV, except for a single mismatch. We conclude that limited sequencing of amplified DNA is a powerful tool for classification as well as molecular epidemiology of foamy viruses.
通过使用与 pol 基因高度保守部分同源的引物进行 PCR 扩增，获得了一段 425bp 的 DNA 片段，以此确定灵长类泡沫病毒之间的关系。所研究的 14 种泡沫病毒的系统发育树反映了它们宿主物种之间的关系：包括猿猴泡沫病毒（SFV）原型 1 和 2（从台湾猕猴分离得到）的亚洲旧世界猴泡沫病毒簇，与包括原型 SFV - 3 和 SFV - 3 菌株 LK - 3（从非洲绿猴，即埃塞俄比亚猕猴分离得到）的非洲旧世界泡沫病毒分离开来。这两个旧世界猴泡沫病毒簇与包括原型 SFV - 6、SFV - 7、SFV cpz（均从黑猩猩分离得到）以及人类泡沫病毒（HFV）的猿类和人类泡沫病毒簇的亲缘关系更远。新世界原型 SFV - 8（从蜘蛛猴，即蛛猴属分离得到）与旧世界簇不同。我们从恒河猴（猕猴）和非洲绿猴分离得到的泡沫病毒分别被归类到亚洲或非洲旧世界猴泡沫病毒簇中。比较了从两名人类淋巴细胞中获得的泡沫病毒序列，其中一人接触过非洲绿猴，另一人接触过培养的 HFV。第一个序列与非洲旧世界猴泡沫病毒簇密切相关，而第二个序列除了一个单碱基错配外，与 HFV 完全相同。我们得出结论，扩增 DNA 的有限测序是泡沫病毒分类以及分子流行病学研究的有力工具。
