Lin F H, Lin C C, Liu H C, Huang Y Y, Wang C Y, Lu C M
Center for Biomedical Engineering, College of Medicine, National Taiwan University, Taipei, Republic of China.
Biomaterials. 1994 Oct;15(13):1087-98.
There is extensive experimental and surgical experience with the use of bone tissue to fill defects in the skeleton, to bridge non-union sites, and to pack defects in bone created from cyst curettage. DP-bioactive glass with a chemical composition of Na2O 8.4%, SiO2 39.6%, P2O5 12% and CaO 40% has been reported as an alternative bone substitute of high mechanical strength, good biocompatibility. and which has a tight bond with living tissue. The bonding layer between DP-bioactive glass and bone tissue was considered to be formed by dissolution of calcium and phosphate ions from the DP-bioactive glass into the surrounding body fluids. The biological hydroxyapatite was suspected to deposit directly onto the bonding layer. In order to confirm the interaction between the DP-bioactive glass and bone tissue, the developed bioactive glass was implanted into rabbit femur condyle for 2-32 weeks. The histological evaluation of DP-bioactive glass as a bone substitute was also investigated in the study. Porous hydroxyapatite bioceramic was used in the control group and the results were compared with those of DP-bioactive glass. The interface between the DP-bioactive glass and bone tissue examined with SEM-EPMA showed that the bioactive glass formed a reaction layer on the surface within 2 weeks after operation and formed a direct bond with natural bone. The elements contained in the bioactive glass apparently interdiffuse with the living bone and biological hydroxyapatite deposited onto the diffusion area, which was proved by EPMA and TEM. After implantation for over 8 weeks, the DP-bioactive glass was gradually biodegraded and absorbed by the living bone. Histological examination using the optical microscope showed that osteocytes grow into the inside of the DP-bioactive glass and the bioactive glass would be expected to be a part of bone.
在使用骨组织填充骨骼缺损、连接骨不连部位以及填充刮除囊肿后形成的骨缺损方面，已有广泛的实验和手术经验。据报道，化学成分（Na2O 8.4%、SiO2 39.6%、P2O5 12%和CaO 40%）的DP生物活性玻璃是一种具有高机械强度、良好生物相容性且与活组织紧密结合的替代骨材料。DP生物活性玻璃与骨组织之间的结合层被认为是由钙和磷酸根离子从DP生物活性玻璃溶解到周围体液中而形成的。怀疑生物羟基磷灰石直接沉积在结合层上。为了证实DP生物活性玻璃与骨组织之间的相互作用，将研发出（制成）的生物活性玻璃植入兔股骨髁2至32周。该研究还对DP生物活性玻璃作为骨替代物进行了组织学评估。对照组使用多孔羟基磷灰石生物陶瓷，并将结果与DP生物活性玻璃的结果进行比较。用扫描电子显微镜-电子探针微分析（SEM-EPMA）检查DP生物活性玻璃与骨组织之间的界面，结果表明，生物活性玻璃在术后2周内在表面形成反应层，并与天然骨形成直接结合。生物活性玻璃中所含元素明显与活骨相互扩散，且生物羟基磷灰石沉积在扩散区域，这一点通过电子探针微分析（EPMA）和透射电子显微镜（TEM）得到了证实。植入8周以上后，DP生物活性玻璃逐渐被活骨生物降解并吸收。使用光学显微镜进行的组织学检查表明，骨细胞长入DP生物活性玻璃内部，并且该生物活性玻璃有望成为骨的一部分。
