西咪替丁对豚鼠回肠发育过程中组胺和二甲双胍诱导反应的影响。

Effect of cimetidine on the histamine and dimaprit induced responses on the guinea pig ileum during development.

Koutsoviti-Papadopoulou M, Kounenis G, Elezoglou V, Kokolis N

Department of Pharmacology, Faculty of Veterinary Medicine, Aristelian University, Thessaloniki, Greece.

Gen Pharmacol. 1993 Jul;24(4):1021-6. doi: 10.1016/0306-3623(93)90182-w.

Abstract

In ileal segments obtained from 1, 15, 30, 45 and 60-day-old guinea pigs, histamine produced a dose-dependent significant contractile response from a threshold concentration of 3.2 x 10(-8) M. 2. The mean contractile responses (at 10(-5) M) of the ileum from the 1, 15, 30 and 45-day-old guinea pigs compared to that from the 60-day-old ones were 73.79, 92.30, 94.07 and 94.41% respectively. 3. Dimaprit (at 10(-5) M) had no effect on the ileum of the 1-day-old guinea pigs, while it produced a significant contractile response on the ileum of the guinea pigs at all other ages. 4. The mean contractile responses of the ileum from the 15, 30 and 45-day-old guinea pigs compared to that from the 60-day-old ones were 74.44, 66.66 and 76.94% respectively. 5. Cimetidine (at 10(-5), 3.2 x 10(-5) and 10(-4) M) significantly potentiated the histamine-induced contraction on the ileum of the 1-day (from 10(-7) to 3.2 x 10(-6) M) and the 15-day-old guinea pigs (at 10(-8) M). 6. On the contrary, cimetidine (at 3.2 x 10(-5) M) significantly inhibited the contractile response of the ileum to dimaprit. 7. In conclusion, the guinea pig ileum appears increasingly sensitive to histamine from the newborn to the 60-day-old ones, but its sensitivity to dimaprit, not detectable in the newborn, fluctuates during development. 8. Cimetidine produces a potentiating effect, which declines with age, on the on the histamine-induced contraction, while it inhibits the dimaprit contractile response on the ileum.

摘要

在从1日龄、15日龄、30日龄、45日龄和60日龄豚鼠获取的回肠段中，组胺从3.2×10⁻⁸ M的阈值浓度开始产生剂量依赖性的显著收缩反应。2. 1日龄、15日龄、30日龄和45日龄豚鼠回肠的平均收缩反应（在10⁻⁵ M时）与60日龄豚鼠相比，分别为73.79%、92.30%、94.07%和94.41%。3. 地马普明（在10⁻⁵ M时）对1日龄豚鼠的回肠无影响，而对所有其他年龄段豚鼠的回肠产生显著的收缩反应。4. 15日龄、30日龄和45日龄豚鼠回肠的平均收缩反应与60日龄豚鼠相比，分别为74.44%、66.66%和76.94%。5. 西咪替丁（在10⁻⁵、3.2×10⁻⁵和10⁻⁴ M时）显著增强组胺对1日龄（从10⁻⁷到3.2×10⁻⁶ M）和15日龄豚鼠回肠（在10⁻⁸ M时）的收缩作用。6. 相反，西咪替丁（在3.2×10⁻⁵ M时）显著抑制回肠对地马普明的收缩反应。7. 总之，豚鼠回肠从新生到60日龄对组胺的敏感性似乎逐渐增加，但其对新生豚鼠中未检测到的地马普明的敏感性在发育过程中波动。8. 西咪替丁对组胺诱导的收缩产生增强作用，该作用随年龄下降，而它抑制回肠对地马普明的收缩反应。