分子伴侣TF55。对称性评估。

The molecular chaperone TF55. Assessment of symmetry.

作者信息

Marco S, Ureña D, Carrascosa J L, Waldmann T, Peters J, Hegerl R, Pfeifer G, Sack-Kongehl H, Baumeister W

机构信息

Centro Nacional de Biotecnología (CSIC), Universidad Autónoma de Madrid, Spain.

出版信息

FEBS Lett. 1994 Mar 21;341(2-3):152-5. doi: 10.1016/0014-5793(94)80447-8.

DOI:10.1016/0014-5793(94)80447-8

PMID:7907992

Abstract

TF55-like factor from Sulfolobus solfataricus was purified to homogeneity and analyzed by electron microscopy and image analysis to determine the symmetries of these particles. Three different procedures were used to analyze the electron micrographs: (1) fuzzy-set based classification of the particles according to their rotational power spectra; (2) multivariate statistical analysis based on singular value decomposition; (3) circular harmonic analysis. Averages obtained from the three methods show unequivocally that the TF55-like complex presents a 9-fold symmetry.

摘要

从嗜热栖热菌中纯化出类TF55因子并使其达到同质，通过电子显微镜和图像分析来确定这些颗粒的对称性。使用了三种不同的程序来分析电子显微照片：(1) 根据颗粒的旋转功率谱进行基于模糊集的分类；(2) 基于奇异值分解的多变量统计分析；(3) 圆谐波分析。从这三种方法获得的平均值明确显示，类TF55复合物呈现出九重对称性。