豚鼠脑的二肽基氨基肽酶活性
Dipeptidyl aminopeptidase activities of guinea-pig brain.
作者信息
Smyth M, O'Cuinn G
机构信息
Department of Biochemistry, University College, Galway, Ireland.
出版信息
Int J Biochem. 1994 Jul;26(7):913-21. doi: 10.1016/0020-711x(94)90085-x.
PMID:7914879
Abstract
- The subcellular distribution of dipeptidyl aminopeptidase activities in guinea-pig brain was investigated. Our studies show that DAP I (Gly-Arg-NH-Mec hydrolase) type activity was found to have an acidic optimum and was associated with the nuclear pellet. 2. No DAP II (Lys-Ala-NH-Mec hydrolase) type activity could be detected. Apparent hydrolysis was mainly due to aminopeptidase activity. 3. DAP III (Arg-Arg-NH-Mec hydrolase) type activity is largely cytoplasmic, but there was evidence of a membrane form associated with the synaptosomes. 4. DAP IV (Gly-Pro-NH-Mec hydrolase) type activity is present on the synaptosomal membrane, and also enriched in the microsomes. A soluble form of Gly-Pro-NH-Mec hydrolase activity is also present in the cytoplasm. Whether this activity is a DAP II or IV type activity is still yet to be determined.
摘要
- 对豚鼠脑中二肽基氨基肽酶活性的亚细胞分布进行了研究。我们的研究表明，发现DAP I（甘氨酰 - 精氨酸 - 氨 - 甲基香豆素水解酶）型活性具有酸性最佳pH值，且与核沉淀相关。2. 未检测到DAP II（赖氨酰 - 丙氨酸 - 氨 - 甲基香豆素水解酶）型活性。明显的水解主要归因于氨肽酶活性。3. DAP III（精氨酰 - 精氨酸 - 氨 - 甲基香豆素水解酶）型活性主要存在于细胞质中，但有证据表明存在与突触体相关的膜形式。4. DAP IV（甘氨酰 - 脯氨酸 - 氨 - 甲基香豆素水解酶）型活性存在于突触体膜上，并且在微粒体中也有富集。细胞质中也存在甘氨酰 - 脯氨酸 - 氨 - 甲基香豆素水解酶活性的可溶性形式。这种活性是DAP II型还是IV型活性仍有待确定。
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