Vague P, Ramahandridona G, Di Campo-Rougerie C, Mahmoud F
Diabete Metab. 1975 Sep;1(3):185-9.
The collection every 15 seconds of the portal effluent of the isolated and perfused rat pancreas permitted the following observations: while the insulin response to tolbutamide (250 or 125 mug/ml) occurred immediately, that to glucose (15 mM), or to 1-leucine (15 mM) was delayed by 60-90 seconds. The response to d-glyceraldehyde occurred slightly but significantly more quickly than that of glucose. The response to alpha-keto-isocaproate was an intense and immediate one. Isovalerate elicited no insulin discharge. These results suggest that d-glucose and l-leucine have to be metabolized to stimulate insulin secretion and that alpha-keto-isocaproate seems to be a direct and specific signal.
每隔15秒收集分离并灌注的大鼠胰腺门静脉流出液，得出以下观察结果：对甲苯磺丁脲（250或125微克/毫升）的胰岛素反应立即出现，而对葡萄糖（15毫摩尔）或1-亮氨酸（15毫摩尔）的反应延迟60 - 90秒。对d-甘油醛的反应比葡萄糖的反应稍快且明显更快。对α-酮异己酸的反应强烈且立即出现。异戊酸未引起胰岛素释放。这些结果表明，d-葡萄糖和l-亮氨酸必须被代谢才能刺激胰岛素分泌，并且α-酮异己酸似乎是一个直接且特定的信号。
