Rao A S, Demetris A J, Qian S, Murase N, Li Y, Fung J J, Starzl T E
Pittsburgh Transplant Institute, University of Pittsburgh Medical Center, PA 15213.
Cell Transplant. 1994 Jul-Aug;3(4):345-8. doi: 10.1177/096368979400300412.
After whole organ transplantation, donor bone marrow-derived cells migrate out of the graft into the recipient, leading to establishment of chimerism, which is the first step towards the subsequent induction of donor-specific tolerance. In routine immunohistochemical staining, monoclonal antibodies specific for heterotopic MHC alleles are used to identify donor and recipient cells. However, it is difficult to detect these cells using this technique in long-term allograft recipients who have a persistently low donor cell population (microchimerism). Because Interferon-gamma (IFN-gamma) is known to induce expression of MHC class I and class II cell surface molecules, we used this cytokine 12-48 h before sacrifice, to facilitate the identification of donor and recipient cells in the tissues of animals transplanted with either liver (B10-->C3H) or bone marrow (LEW-->BN). In long-term allograft recipients, the use of IFN-gamma for as briefly as 12 h prior to sacrifice, results in marked upregulation of class I and class II antigens, leading to easy identification of ubiquitously distributed low numbers of donor cells.
在全器官移植后，供体骨髓来源的细胞从移植物中迁移至受体体内，导致嵌合体的形成，这是后续诱导供体特异性耐受的第一步。在常规免疫组织化学染色中，针对异位MHC等位基因的单克隆抗体用于识别供体细胞和受体细胞。然而，对于长期同种异体移植受体中供体细胞数量持续较低（微嵌合体）的情况，使用该技术难以检测到这些细胞。由于已知干扰素-γ（IFN-γ）可诱导MHC I类和II类细胞表面分子的表达，我们在处死动物前12 - 48小时使用这种细胞因子，以促进在接受肝脏（B10→C3H）或骨髓（LEW→BN）移植的动物组织中识别供体细胞和受体细胞。在长期同种异体移植受体中，在处死前仅使用12小时的IFN-γ，就会导致I类和II类抗原显著上调，从而易于识别广泛分布的少量供体细胞。
