人TAFII30存在于一种独特的TFIID复合物中，是雌激素受体转录激活所必需的。

Human TAFII30 is present in a distinct TFIID complex and is required for transcriptional activation by the estrogen receptor.

作者信息

Jacq X, Brou C, Lutz Y, Davidson I, Chambon P, Tora L

机构信息

Laboratoire de Génétique Moléculaire des Eucaryotes, Centre National de la Recherche Scientifique, Faculté de Médecine, Strasbourg, France.

出版信息

Cell. 1994 Oct 7;79(1):107-17. doi: 10.1016/0092-8674(94)90404-9.

DOI:10.1016/0092-8674(94)90404-9

PMID:7923369

Abstract

We showed previously that coactivators mediating stimulation by different activators were associated with the TATA-binding protein (TBP) in distinct TFIID complexes. We have characterized a human TBP-associated factor (TAF), hTAFII30, associated with a subset of TFIID complexes. hTAFII30 interacts with the AF-2-containing region E of the human estrogen receptor (ER), but not with ER AF-1 or VP16. An antibody against hTAFII30 inhibited transcriptional stimulation by the ER AF-2 without affecting basal or VP16-activated transcription and allowed the separation of TFIID complex(es) containing hTAFII30 from complexes mediating the activity of VP16. These results directly demonstrate the existence of functionally distinct TFIID populations that share common TAFIIs but differ in specific TAFIIs.

摘要

我们之前表明，介导不同激活因子刺激作用的共激活因子，在不同的TFIID复合物中与TATA结合蛋白（TBP）相关联。我们已经鉴定出一种人类TBP相关因子（TAF），即hTAFII30，它与一部分TFIID复合物相关。hTAFII30与人雌激素受体（ER）含AF-2的区域E相互作用，但不与ER的AF-1或VP16相互作用。一种针对hTAFII30的抗体抑制了ER AF-2介导的转录刺激，而不影响基础转录或VP16激活的转录，并使得含有hTAFII30的TFIID复合物与介导VP16活性的复合物得以分离。这些结果直接证明了功能不同的TFIID群体的存在，这些群体共享共同的TBP相关因子（TAFIIs），但在特定的TAFIIs上存在差异。