人垂体腺瘤中编码激素原转化酶（PC2和PC3）的信使核糖核酸的表达模式

Expression patterns of messenger ribonucleic acids encoding prohormone convertases (PC2 and PC3) in human pituitary adenomas.

作者信息

Hashimoto K, Koga M, Kouhara H, Arita N, Hayakawa T, Kishimoto T, Sato B

机构信息

Department of Medicine III, Osaka University Hospital, Suita-shi, Japan.

出版信息

Clin Endocrinol (Oxf). 1994 Aug;41(2):185-91. doi: 10.1111/j.1365-2265.1994.tb02528.x.

DOI:10.1111/j.1365-2265.1994.tb02528.x

PMID:7923822

Abstract

OBJECTIVE

Prohormone convertases (PC2 and PC3) have been reported to play an important role for prohormone processing in rodent pituitaries. However, expression of mRNAs encoding these enzymes has not been characterized in human pituitaries. In addition, altered or insufficient prohormone processing has been reported in some human pituitary adenomas. Thus, the expression patterns of these mRNAs in non-tumorous and tumorous human pituitaries should be examined.

DESIGN

Total RNAs were extracted from non-tumorous or tumorous human pituitaries to analyse PC2 and PC3 mRNA expression.

SAMPLES

Five ACTH producing adenomas, 11 GH producing adenomas, one PRL producing adenoma and five non-functioning adenomas were obtained at surgery. Two non-tumorous pituitaries were also included in this study.

MEASUREMENTS

The contents were quantitatively measured by Northern blot analysis using rat PC3 cDNA or human PC2 cDNA as a probe. The method was also developed for the detection of PC2 mRNA by Southern blot analysis of reverse transcriptase-polymerase chain reaction (RT-PCR) products.

RESULTS

PC2 and PC3 mRNAs in non-tumorous samples were detected by Northern blot analysis whereas their contents in tumorous samples varied from high levels to undetectable. Marked variation of PC3 mRNA levels was observed among GH producing adenomas. ACTH producing adenomas were observed to express PC3 mRNA. Northern blot analysis also revealed that PC2 mRNA levels in ACTH producing adenomas were low except for one sample. PC2 mRNA expression in GH producing adenomas was confirmed by Southern blot analysis of RT-PCR products. This procedure also confirmed the various levels of PC2 mRNA expression among ACTH producing adenomas.

CONCLUSION

The expression of PC2 and PC3 mRNA in human pituitaries has been confirmed. However, their expression has been observed to vary quantitatively and not to be restricted to certain types of pituitary cells.

摘要

目的

据报道，激素原转化酶（PC2和PC3）在啮齿动物垂体的激素原加工过程中发挥重要作用。然而，编码这些酶的mRNA在人垂体中的表达尚未得到表征。此外，据报道，在一些人垂体腺瘤中存在激素原加工改变或不足的情况。因此，应检测这些mRNA在非肿瘤性和肿瘤性人垂体中的表达模式。

设计

从非肿瘤性或肿瘤性人垂体中提取总RNA，以分析PC2和PC3 mRNA的表达。

样本

手术中获得了5例促肾上腺皮质激素（ACTH）分泌型腺瘤、11例生长激素（GH）分泌型腺瘤、1例催乳素（PRL）分泌型腺瘤和5例无功能腺瘤。本研究还纳入了2个非肿瘤性垂体。

测量

使用大鼠PC3 cDNA或人PC2 cDNA作为探针，通过Northern印迹分析对含量进行定量测量。还开发了通过逆转录聚合酶链反应（RT-PCR）产物的Southern印迹分析来检测PC2 mRNA的方法。

结果

通过Northern印迹分析在非肿瘤性样本中检测到PC2和PC3 mRNA，而它们在肿瘤性样本中的含量从高水平到无法检测不等。在GH分泌型腺瘤中观察到PC3 mRNA水平存在明显差异。观察到ACTH分泌型腺瘤表达PC3 mRNA。Northern印迹分析还显示，除了一个样本外，ACTH分泌型腺瘤中的PC2 mRNA水平较低。通过RT-PCR产物的Southern印迹分析证实了GH分泌型腺瘤中PC2 mRNA的表达。该方法还证实了ACTH分泌型腺瘤中PC2 mRNA表达的不同水平。