Fujii J T
Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan 48201.
Exp Neurol. 1994 Sep;129(1):155-62. doi: 10.1006/exnr.1994.1156.
Chick Edinger-Westphal neurons form large, calyciform synaptic terminals, also known as calyces, on a subpopulation of ciliary ganglion neurons in vivo. This distinctive morphology is mimicked by contacts formed between Edinger-Westphal and ciliary ganglion neurons in culture. The work reported here finds that calyx-like contacts in culture are immunoreactive for the two vesicle proteins SV-2 and synaptotagmin, supporting the hypothesis that these contacts are synaptic terminals developing in vitro. When Edinger-Westphal neurons are cultured with ciliary ganglion neurons, calyx-like contacts are three times more frequent than when Edinger-Westphal neurons are cultured inappropriately with sympathetic neurons. Furthermore, calyx-like contacts are rare in cultures of ciliary ganglion neurons alone, even though ciliary ganglion neurons are known to functionally innervate themselves in culture. These results suggest that calyx morphogenesis is favored when Edinger-Westphal neurons interact specifically with ciliary ganglion neurons and discouraged when they interact with inappropriate neurons. That this specificity is expressed in culture, in the absence of normal tissue topography and patterned activity, adds to the evidence that direct surface interactions between target cells and growing neurites are involved in the morphogenesis of synaptic terminals in vivo.
鸡动眼神经副核（Edinger-Westphal）神经元在体内会在睫状神经节神经元的一个亚群上形成大的、杯状突触终末，也称为杯状体。这种独特的形态在体外培养的动眼神经副核神经元与睫状神经节神经元之间形成的接触中也有体现。本文报道的研究发现，体外培养中的杯状接触对两种囊泡蛋白SV-2和突触结合蛋白具有免疫反应性，支持了这些接触是体外发育的突触终末这一假说。当动眼神经副核神经元与睫状神经节神经元共同培养时，杯状接触的出现频率是动眼神经副核神经元与交感神经元不适当共同培养时的三倍。此外，单独培养睫状神经节神经元时杯状接触很少见，尽管已知睫状神经节神经元在培养中会对自身进行功能性支配。这些结果表明，当动眼神经副核神经元与睫状神经节神经元特异性相互作用时，杯状体形态发生受到促进，而当它们与不适当的神经元相互作用时则受到抑制。这种特异性在体外培养中表现出来，此时不存在正常的组织拓扑结构和模式化活动，这进一步证明了靶细胞与生长中的神经突之间的直接表面相互作用参与了体内突触终末的形态发生。
