Brandeis J M, Sayegh M H, Gallon L, Blumberg R S, Carpenter C B
Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.
Gastroenterology. 1994 Nov;107(5):1537-42. doi: 10.1016/0016-5085(94)90560-6.
BACKGROUND/AIMS: Intestinal epithelial cells present protein antigens to primed T cells in vitro. The aim of this study was to investigate whether intestinal epithelial cells present peptide antigens in vitro and in vivo after oral administration.
Small intestinal epithelial cells from naive LEW (RT1) rats pulsed in vitro with a synthetic immunogenic major histocompatibility complex allopeptide, RT1.Du beta 20-44, or in vivo by oral administration of the peptide were tested for their ability to induce specific proliferation of LEW T cells primed in vivo to RT1.Du beta 20-44.
In vitro pulsed intestinal epithelial cells induced specific proliferation of RT1.Du beta 20-44-primed T cells. Intestinal epithelial cells isolated from LEW ras that received a single oral dose of RT1.Du beta 20-44 18 hours earlier also induced specific proliferation of RT1.Du beta 20-44-primed LEW T cells. Furthermore, epithelial cells harvested from LEW rats that received WF (RT1u) splenocytes orally 18 hours earlier induced specific proliferation of RT1.Du beta 20-44-primed LEW T cells.
Intestinal epithelial cells take up processed alloantigen in vitro and in vivo for presentation as peptides to primed T cells. These observations provide a novel approach to study the role of the intestinal immune system in immune regulation in vivo.
背景/目的：肠上皮细胞可在体外将蛋白质抗原呈递给致敏T细胞。本研究旨在探讨肠上皮细胞在体外以及口服给药后在体内是否能呈递肽抗原。
用合成的免疫原性主要组织相容性复合体异源肽RT1.Duβ20 - 44在体外脉冲处理来自未致敏LEW（RT1）大鼠的小肠上皮细胞，或通过口服该肽在体内处理，然后检测这些细胞诱导在体内已致敏于RT1.Duβ20 - 44的LEW T细胞特异性增殖的能力。
体外脉冲处理的肠上皮细胞可诱导RT1.Duβ20 - 44致敏T细胞的特异性增殖。从18小时前单次口服剂量RT1.Duβ20 - 44的LEW大鼠分离的肠上皮细胞也可诱导RT1.Duβ20 - 44致敏的LEW T细胞的特异性增殖。此外，从18小时前口服WF（RT1u）脾细胞的LEW大鼠收获的上皮细胞可诱导RT1.Duβ20 - 44致敏的LEW T细胞特异性增殖。
肠上皮细胞在体外和体内摄取加工后的同种异体抗原，以肽的形式呈递给致敏T细胞。这些观察结果为研究肠道免疫系统在体内免疫调节中的作用提供了一种新方法。
