编码14-3-3同源物的秀丽隐杆线虫cDNA的分离与序列分析。

Isolation and sequence analysis of a Caenorhabditis elegans cDNA which encodes a 14-3-3 homologue.

作者信息

Wang W, Shakes D C

机构信息

Department of Biology and Institute of Molecular Biology, University of Houston, TX 77204-5513.

出版信息

Gene. 1994 Sep 30;147(2):215-8. doi: 10.1016/0378-1119(94)90068-x.

DOI:10.1016/0378-1119(94)90068-x

PMID:7926802

Abstract

We report the cloning of the Caenorhabditis elegans homologue of a 14-3-3 protein-encoding gene which is located within the unc-22 gene cluster on chromosome IV. Sequence analysis reveals that the cDNA-encoded product is 78% identical to both the Drosophila melanogaster and bovine 14-3-3 proteins. Our cDNA hybridizes to at least three major transcripts of 1.5, 1.35 and 0.9 kb, which are all more abundant in fertile hermaphrodites than in those lacking germ cells.

摘要

我们报道了位于线虫IV号染色体unc-22基因簇内的一个编码14-3-3蛋白的基因的秀丽隐杆线虫同源基因的克隆。序列分析表明，该cDNA编码的产物与果蝇和牛的14-3-3蛋白均有78%的同源性。我们的cDNA与至少三种主要转录本杂交，大小分别为1.5、1.35和0.9 kb，这些转录本在可育雌雄同体中的丰度均高于缺乏生殖细胞的个体。