Wagner D R, Heinrich D
International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Infect Immun. 1994 Oct;62(10):4320-4. doi: 10.1128/iai.62.10.4320-4324.1994.
In gram-negative sepsis, the activation of polymorphonuclear leukocytes (PMN) by lipopolysaccharide (LPS) and the resulting production of superoxide and other oxygen radicals may be an important cause of tissue damage. A suppression of the PMN response to LPS stimulation would be therapeutically beneficial. The aim of this study was to determine whether different polyclonal immunoglobulins (Igs; 5S-Ig, 7S-Ig, and 19S-Ig) influence the PMN response to LPS of Salmonella enteritidis in vitro. The respiratory burst activity of PMN was measured with luminol-enhanced chemiluminescence. After addition of a 5S-Ig solution containing F(ab')2 fragments of IgG and a 19S-Ig solution containing 12% polyclonal IgM, luminol-enhanced chemiluminescence was reduced by 27% (P < 0.05) and 46% (P < 0.005), respectively. However, after addition of a 7S-Ig solution containing polyclonal IgG, luminol-enhanced chemiluminescence was increased fourfold (P < 0.05). The results suggest that the influence of polyclonal Igs on PMN response to LPS stimulation is dependent on the Ig class, F(ab')2 fragments of IgG and IgM leading to LPS neutralization and IgG leading to the production of potentially toxic oxygen radicals.
在革兰氏阴性菌败血症中，脂多糖（LPS）激活多形核白细胞（PMN）并由此产生超氧化物和其他氧自由基可能是组织损伤的一个重要原因。抑制PMN对LPS刺激的反应在治疗上可能有益。本研究的目的是确定不同的多克隆免疫球蛋白（Igs；5S-Ig、7S-Ig和19S-Ig）是否在体外影响PMN对肠炎沙门氏菌LPS的反应。用鲁米诺增强化学发光法测量PMN的呼吸爆发活性。加入含有IgG的F(ab')2片段的5S-Ig溶液和含有12%多克隆IgM的19S-Ig溶液后，鲁米诺增强化学发光分别降低了27%（P<0.05）和46%（P<0.005）。然而，加入含有多克隆IgG的7S-Ig溶液后，鲁米诺增强化学发光增加了四倍（P<0.05）。结果表明，多克隆Igs对PMN对LPS刺激反应的影响取决于Ig类别，IgG的F(ab')2片段和IgM导致LPS中和，而IgG导致产生潜在有毒的氧自由基。
