钙非依赖性蛋白激酶C在NG 108-15细胞分化中的表达与调控

Expression and regulation of calcium-independent protein kinase C in NG 108-15 cell differentiation.

作者信息

Battaini F, Pascale A, Lucchi L, Racchi M, Bergamaschi S, Parenti M, Wetsel W C, Govoni S, Trabucchi M

机构信息

Department of Experimental Medicine and Biochemical Sciences, University of Roma Tor Vergata, Italy.

出版信息

Biochem Biophys Res Commun. 1994 Sep 30;203(3):1423-31. doi: 10.1006/bbrc.1994.2344.

DOI:10.1006/bbrc.1994.2344

PMID:7945290

Abstract

Protein, mRNA and activity levels of the calcium-independent protein kinase C (nPKC) isoenzymes were examined in NG108-15 cells. Western blot analyses reveal that proliferating NG 108-15 cells express the delta, epsilon, and eta, but not the theta species. The atypical species PKC zeta was also detected. Differentiation of these cells with dibutyryl cAMP was associated with increase in the levels of PKC epsilon, with no significant changes in its steady-state mRNA levels. The levels of the other isoforms were not altered by the differentiated state. Similarly, no changes in nPKC activity were discerned in either the soluble or particulate fractions when histone or other proteins were used as substrates. These data suggest that the PKC epsilon isoform may be important for the production and maintenance of the differentiated state in NG 108-15 cells.

摘要

在NG108-15细胞中检测了非钙依赖性蛋白激酶C（nPKC）同工酶的蛋白质、mRNA和活性水平。蛋白质免疫印迹分析显示，增殖的NG 108-15细胞表达δ、ε和η亚型，但不表达θ亚型。还检测到了非典型亚型PKC ζ。这些细胞用二丁酰环磷腺苷（dibutyryl cAMP）诱导分化与PKC ε水平升高相关，其稳态mRNA水平无显著变化。其他同工型的水平不受分化状态的影响。同样，当使用组蛋白或其他蛋白质作为底物时，无论是可溶性部分还是颗粒部分，nPKC活性均无明显变化。这些数据表明，PKC ε同工型可能对NG 108-15细胞分化状态的产生和维持很重要。