采用改进的磷脂分析方法检测人肠黏膜中的血浆乙醇胺磷脂。
Plasmenylethanolamine in human intestinal mucosa detected by an improved method for analysis of phospholipid.
作者信息
Nakanishi K, Yasugi E, Morita H, Dohi T, Oshima M
机构信息
Division of Biochemistry and Nutrition, International Medical Center of Japan, Tokyo.
出版信息
Biochem Mol Biol Int. 1994 Jun;33(3):457-62.
Analysis of phospholipid and their fatty acid composition of human intestinal mucosa was performed by an method elaborated to analyze the limited amount of sample with 2-dimensional TLC followed by lipid-phosphorus determination. Using this method, plasmenylethanolamine was detected in human intestinal mucosa and accounted for about 7% of phospholipid in small and large intestinal mucosa. The amounts of polyunsaturated fatty acids of phosphatidylethanolamine were higher than those of other phosphoglycerides in intestinal mucosa, hence, inflammation-related eicosanoids may originate from ethanolamine containing phospholipid.
采用一种精心设计的方法对人肠黏膜的磷脂及其脂肪酸组成进行分析，该方法通过二维薄层色谱法对少量样品进行分析，随后进行脂质磷测定。使用该方法，在人肠黏膜中检测到了缩醛磷脂酰乙醇胺，其在小肠和大肠黏膜磷脂中约占7%。肠黏膜中磷脂酰乙醇胺的多不饱和脂肪酸含量高于其他磷酸甘油酯，因此，与炎症相关的类花生酸可能源自含乙醇胺的磷脂。
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