Capillary electrophoretic examination of underivatized O-linked and N-linked oligosaccharide mixtures and immunoglobulin G antibody-released oligosaccharide libraries.

A procedure for the analysis of mixtures of underivatized, cleaved O-linked and N-linked oligosaccharides by capillary electrophoresis has been developed. The species of interest are separated by two borate-based buffer systems with an uncoated capillary. The procedure is applied to hydrazinolysis-released oligosaccharides from mouse, rat, sheep and human IgG protein samples which are examined within 6 minutes. Selectivity with respect to sample variation is demonstrated by analysis of thermally stressed samples.