Schulz M K, Handa R J, Tillotson G L, Castro A J
Department of Cell Biology, Neurobiology and Anatomy, Loyola University School of Medicine, Maywood, Illinois.
Exp Neurol. 1994 Oct;129(2):321-9. doi: 10.1006/exnr.1994.1174.
Using anatomical and electrophysiological methods, previous studies from this laboratory demonstrated that fetal neocortical block transplants placed into the excitotoxically ablated cortex of adult rats can establish functional connections with the host central nervous system. In order to further examine functional integration at the level of genomic activation/molecular signaling systems, transplants were analyzed for the presence of c-fos mRNA and Fos protein-labeled cells following a 20-min exposure of the animal to a novel open-field environment. This behavioral testing paradigm, which previously was shown to induce expression of c-fos mRNA in several brain regions including the sensorimotor cortex, was administered at 3 months after grafting. The transplants were found to express c-fos mRNA as well as c-fos protein following novelty exposure. These labeled cells were distributed primarily along the peripheral margin of the grafts. This study therefore suggests that at least some neurons within transplants become integrated with the host pathways at the level of molecular signal transduction.
本实验室先前的研究运用解剖学和电生理学方法证明，植入成年大鼠经兴奋性毒素损毁的皮质中的胎儿新皮质块移植体能与宿主中枢神经系统建立功能连接。为了进一步检测基因组激活/分子信号系统水平上的功能整合情况，在动物暴露于新的旷场环境20分钟后，分析移植体中c-fos mRNA和Fos蛋白标记细胞的存在情况。这种行为测试范式先前已被证明可在包括感觉运动皮质在内的多个脑区诱导c-fos mRNA的表达，在移植后3个月进行。发现移植体在暴露于新环境后表达c-fos mRNA以及c-fos蛋白。这些标记细胞主要分布在移植体的周边边缘。因此，本研究表明，移植体内至少一些神经元在分子信号转导水平上与宿主通路实现了整合。
