用于检测微生物细胞相关和细胞外蛋白酶活性的包被珠分析法。
Coated-bead assay for microbial cell-associated and extracellular protease activity.
作者信息
Ascencio F, Wadström T
机构信息
Department of Marine Pathology, Center for Biological Research, La Paz, Mexico.
出版信息
Microbios. 1994;79(320):139-44.
PMID:7968666
Abstract
An assay utilizing latex beads coated with substrates of 125I-labelled connective tissue proteins is described as an alternative method for quantitating cell-associated and extracellular proteolytic activity of micro-organisms. Proteolytic activity is a function of the amount of 125I-labelled peptides released into the incubation mixture.
摘要
描述了一种利用涂有125I标记结缔组织蛋白底物的乳胶珠的测定方法，作为定量微生物细胞相关和细胞外蛋白水解活性的替代方法。蛋白水解活性是释放到孵育混合物中的125I标记肽量的函数。
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