从野生中国小鼠中克隆莫洛尼氏鼠白血病病毒（MMTV）到质粒中：病毒长末端重复序列（LTR）的测序

Cloning in a plasmid of an MMTV from a wild Chinese mouse: sequencing of the viral LTR.

作者信息

Xu L, Haga S, Imai S, Sarkar N H

机构信息

Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta 30912.

出版信息

Virus Res. 1994 Aug;33(2):167-78. doi: 10.1016/0168-1702(94)90053-1.

DOI:10.1016/0168-1702(94)90053-1

PMID:7975881

Abstract

Plasmid subcloning by conventional techniques of full length exogenous mouse mammary viruses (MMTV) has not been realized because of the involvement of host-mediated structural changes in the viral gag gene. To circumvent this problem, an alternative subcloning method, excision of phagemid (pBluescript SK) from lambda ZAP II, was successfully used to subclone a novel exogenous MMTV (JYG-MMTV) provirus fragment containing an intact gag gene. Sequence analysis revealed that the LTR of this virus is significantly different from the LTR of C3H-MMTV in the U3 region.

摘要

由于宿主介导的病毒gag基因结构变化的影响，通过常规技术对全长外源性小鼠乳腺病毒（MMTV）进行质粒亚克隆尚未实现。为了解决这个问题，一种替代的亚克隆方法，即从λZAP II中切除噬菌粒（pBluescript SK），成功地用于亚克隆一个包含完整gag基因的新型外源性MMTV（JYG-MMTV）前病毒片段。序列分析表明，该病毒的LTR在U3区域与C3H-MMTV的LTR有显著差异。