Rososhansky S, Szymanski I O
Blood Transfusion Service, University of Massachusetts Medical Center, Worcester 01655.
Am J Hematol. 1994 Dec;47(4):312-5. doi: 10.1002/ajh.2830470412.
We isolated subpopulations of normal blood platelets from platelet rich plasma (PRP) and, in parallel, from platelet rich saline (PRS) on the basis of their buoyant densities. The MPV, being smallest in the lightest subpopulation, correlated significantly with platelet buoyant density (P < 0.002). In agreement with previous reports, we showed that the least dense platelet subpopulation, separated from PRP, had a high level of PA-IgG (3,486 mol/plt). This value was significantly greater (P < 0.02) than PA-IgG in the lightest subpopulation separated from PRS (693 mol/plt). We believe that the procedure of separating platelets from PRP artificially increased PA-IgG in the least dense subpopulation. Our data showed that when subpopulations were prepared from PRS, PA-IgG correlated with platelet buoyant density (P < 0.002), and that the subpopulation with the highest density had the highest level of PA-IgG.
我们基于血小板的漂浮密度，从富血小板血浆（PRP）以及与之平行的富血小板生理盐水（PRS）中分离出正常血小板亚群。平均血小板体积（MPV）在最轻的亚群中最小，与血小板漂浮密度显著相关（P < 0.002）。与先前的报道一致，我们发现从PRP中分离出的密度最小的血小板亚群具有高水平的血小板相关免疫球蛋白G（PA-IgG，3,486摩尔/血小板）。该值显著高于从PRS中分离出的最轻亚群中的PA-IgG（693摩尔/血小板）（P < 0.02）。我们认为从PRP中分离血小板的操作人为地增加了密度最小亚群中的PA-IgG。我们的数据表明，当从PRS制备亚群时，PA-IgG与血小板漂浮密度相关（P < 0.002），并且密度最高的亚群具有最高水平的PA-IgG。
