乳酸克鲁维酵母ERD1同源物的克隆与DNA序列
Cloning and DNA sequence of a Kluyveromyces lactis ERD1 homologue.
作者信息
Dean N
机构信息
Department of Biochemistry and Cell Biology, State University of New York at Stony Brook 11794-5215.
出版信息
Yeast. 1994 Aug;10(8):1117-24. doi: 10.1002/yea.320100814.
The ERD1 gene product is required for the correct localization of soluble proteins that normally reside in the endoplasmic reticulum (ER). Cell lacking ERD1 secrete resident ER proteins and, in addition, exhibit defects in the processing of glycoproteins. Here, the molecular characterization of the Kluyveromyces lactis ERD1 homologue is described. A comparison of the predicted sequences of the Saccharomyces cerevisiae and K. lactis Erd1 proteins indicates that they are about 30% identical and 50% similar in sequence. Despite low sequence identity, these proteins are predicted to be conserved structurally. Furthermore, the K. lactis protein can functionally complement an S. cerevisiae mutant containing a deletion of the entire ERD1 gene, indicating these two proteins are functional homologues.
ERD1基因产物是正常驻留在内质网（ER）中的可溶性蛋白质正确定位所必需的。缺乏ERD1的细胞会分泌内质网驻留蛋白，此外，在糖蛋白加工方面也存在缺陷。在此，描述了乳酸克鲁维酵母ERD1同源物的分子特征。酿酒酵母和乳酸克鲁维酵母Erd1蛋白预测序列的比较表明，它们的序列约30%相同，50%相似。尽管序列同一性较低，但预计这些蛋白质在结构上是保守的。此外，乳酸克鲁维酵母蛋白可以在功能上互补含有整个ERD1基因缺失的酿酒酵母突变体，表明这两种蛋白质是功能同源物。
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