使用单克隆抗体对人触珠蛋白进行免疫亲和纯化。
Immunoaffinity purification of human haptoglobin using monoclonal antibodies.
作者信息
Katnik I, Jadach J
机构信息
Department of Chemistry, Medical Academy, Wrocław, Poland.
出版信息
Arch Immunol Ther Exp (Warsz). 1993;41(5-6):303-8.
Two monoclonal antibodies, coupled to Sepharose 4B, were used for rapid isolation of the human haptoglobin in a single chromatographic step. The purity of haptoglobin was judged by SDS polyacrylamide gel electrophoresis, immunoblotting, immunoelectrophoresis and size exclusion high performance liquid chromatography. Moreover, the preparation showed ability to form active complexes with hemoglobin, Concanavalin A and specific antibody. Polyclonal goat antiserum produced with the obtained haptoglobin preparation formed one precipitin line with whole human serum. On the other hand, monoclonal antibodies can be purified by means of haptoglobin coupled to matrix.
两种与琼脂糖凝胶4B偶联的单克隆抗体被用于在单一色谱步骤中快速分离人触珠蛋白。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、免疫印迹、免疫电泳和尺寸排阻高效液相色谱法来判断触珠蛋白的纯度。此外，该制剂显示出与血红蛋白、伴刀豆球蛋白A和特异性抗体形成活性复合物的能力。用所得触珠蛋白制剂产生的山羊多克隆抗血清与整个人血清形成一条沉淀线。另一方面，单克隆抗体可通过与基质偶联的触珠蛋白进行纯化。
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