C3a和C5A对人中性粒细胞的激活作用。对形态变化、趋化性、分泌及呼吸爆发影响的比较。

Activation of human neutrophils by C3a and C5A. Comparison of the effects on shape changes, chemotaxis, secretion, and respiratory burst.

作者信息

Ehrengruber M U, Geiser T, Deranleau D A

机构信息

Theodor Kocher Institute, University of Bern, Switzerland.

出版信息

FEBS Lett. 1994 Jun 13;346(2-3):181-4. doi: 10.1016/0014-5793(94)00463-3.

DOI:10.1016/0014-5793(94)00463-3

PMID:8013630

Abstract

The effects of anaphylatoxin C3a on human neutrophils were studied in comparison with C5a. Both peptides induced a transient shape change response and a respiratory burst. In both cases C3a was 50- to 100-times less potent than C5a. A marked chemotactic response with bimodal concentration dependence was obtained with C5a, but no neutrophil chemotaxis was observed with C3a. Repeated stimulation led to homologous desensitization of shape changes and respiratory burst but no cross-desensitization, indicating that the two anaphylatoxins act through separate receptors. The lack of chemotactic activity suggests that C3a is not involved in neutrophil recruitment into infected or inflamed tissues.

摘要

将过敏毒素C3a与C5a相比较，研究了其对人中性粒细胞的作用。两种肽均诱导了短暂的形态变化反应和呼吸爆发。在这两种情况下，C3a的效力均比C5a低50至100倍。C5a产生了具有双峰浓度依赖性的明显趋化反应，但未观察到C3a引起的中性粒细胞趋化作用。重复刺激导致形态变化和呼吸爆发的同源脱敏，但未出现交叉脱敏，这表明两种过敏毒素通过不同的受体起作用。缺乏趋化活性表明C3a不参与中性粒细胞募集到感染或发炎组织的过程。