Lipka E, Ganowiak Z
Katedry i Zakładu Bromatologii Akademii Medycznej, Gdańsku.
Rocz Panstw Zakl Hig. 1993;44(2-3):151-8.
The sterilization effect was analysed on the nutritional value of two protein types (milk/casein) and fish (cod). By chemical-biological method (determination of digestibility, assimilate-ability and growth yield) the level of accessible methionine and cysteine, and taurine level as a metabolite of sulphur-containing amino acids were determined in the liver and urine of experimental animals. Cod protein and casein subjected to technological processing showed lower digestibility, assimilate-ability and growth yield as compared with raw protein. The serum level of biologically active sulphur-containing amino acids methionine and cysteine was lower also. A significant correlation was found between the results of the biological assessment of the nutritional value of protein, and the levels of methionine and cysteine in diet on the one hand, and the content of taurine in the liver and urine of rats, on the other hand. The possibility of assessment of the nutritional value of protein based on urinary taurine level is a simple and non-invasive method based on the metabolism of sulphur-containing amino acids in the organism of humans and animals which encourages for further studies.
分析了两种蛋白质类型（牛奶/酪蛋白）和鱼类（鳕鱼）经杀菌处理后的营养价值。通过化学生物学方法（测定消化率、同化率和生长产量），测定了实验动物肝脏和尿液中可利用蛋氨酸和半胱氨酸的水平，以及作为含硫氨基酸代谢产物的牛磺酸水平。经过工艺加工的鳕鱼蛋白和酪蛋白与未加工的蛋白相比，消化率、同化率和生长产量较低。具有生物活性的含硫氨基酸蛋氨酸和半胱氨酸的血清水平也较低。一方面，蛋白质营养价值的生物学评估结果与饮食中蛋氨酸和半胱氨酸的水平之间，另一方面，大鼠肝脏和尿液中牛磺酸的含量之间，均发现了显著相关性。基于尿牛磺酸水平评估蛋白质营养价值的可能性是一种基于人和动物体内含硫氨基酸代谢的简单且非侵入性的方法，这鼓励了进一步的研究。
