从墨西哥流浪蜘蛛（蜘蛛目：栉足蛛科）的多组分毒液中纯化毒性肽并确定CSTX-1的氨基酸序列。

Purification of toxic peptides and the amino acid sequence of CSTX-1 from the multicomponent venom of Cupiennius salei (Araneae:Ctenidae).

作者信息

Kuhn-Nentwig L, Schaller J, Nentwig W

机构信息

Institute of Zoology, University of Bern, Switzerland.

出版信息

Toxicon. 1994 Mar;32(3):287-302. doi: 10.1016/0041-0101(94)90082-5.

DOI:10.1016/0041-0101(94)90082-5

PMID:8016851

Abstract

The venom of the wandering spider Cupiennius salei was analysed biochemically by gel filtration, cation exchange chromatography, RP-HPLC, IEF, SDS-PAGE and TLC-electrophoresis. The native venom contains high levels of Na+, K+, Ca2+, histamine and taurine. It shows considerable activity of hyaluronidase, but not proteolytic activity. Thirteen peptides (CSTX-1 to CSTX-13) with an apparent mol. wt between 2.6 and 12.5 kDa causing differently strong toxic, effects were purified. Toxicity data of the crude venom (insects and mouse) are given and compared with the toxicity of CSTX-1, which causes most of the crude venom's toxicity. CSTX-1 has a mol. wt of 8352.6 and its amino acid sequence of 74 amino acids is given.

摘要

通过凝胶过滤、阳离子交换色谱法、反相高效液相色谱法、等电聚焦、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和薄层色谱电泳对游走蛛（Cupiennius salei）的毒液进行了生化分析。天然毒液含有高水平的钠离子、钾离子、钙离子、组胺和牛磺酸。它具有相当强的透明质酸酶活性，但没有蛋白水解活性。纯化出了13种表观分子量在2.6至12.5 kDa之间的肽（CSTX-1至CSTX-13），它们会产生不同程度的强烈毒性作用。给出了粗毒液对昆虫和小鼠的毒性数据，并与造成粗毒液大部分毒性的CSTX-1的毒性进行了比较。CSTX-1的分子量为8352.6，并给出了其74个氨基酸的氨基酸序列。