Marshall G M, Cheung B, Stacey K P, Norris M D, Haber M
Children's Leukaemia and Cancer Research Centre, Prince of Wales Children's Hospital, Randwick, Sydney, Australia.
Anticancer Res. 1994 Mar-Apr;14(2A):437-41.
Human neuroblastoma (NB) cell lines treated with all-trans retinoic acid (RA) differentiate in culture, forming neurites and exhibiting growth arrest. We treated 5 human NB cell lines with RA, and observed a 2-5 fold induction of retinoic acid receptor alpha (RAR alpha) mRNA expression in 4 of the 5 cell lines, as an early cellular response. Induction of RAR alpha expression was specific for RA among several differentiating agents tested. RAR alpha mRNA expression in 13 primary neuroblastoma tumor samples was 3 fold higher in localised compared with advanced tumors (p < 0.05). RAR alpha expression may be necessary for the effects of RA on NB cells in vitro and reduced expression of this gene in vivo may contribute to the process of NB tumorigenesis.
用全反式维甲酸（RA）处理的人神经母细胞瘤（NB）细胞系在培养中发生分化，形成神经突并表现出生长停滞。我们用RA处理了5种人NB细胞系，并观察到5种细胞系中有4种出现视黄酸受体α（RARα）mRNA表达2 - 5倍的诱导，这是一种早期细胞反应。在所测试的几种分化剂中，RARα表达的诱导对RA具有特异性。13个原发性神经母细胞瘤肿瘤样本中，局限性肿瘤的RARα mRNA表达比晚期肿瘤高3倍（p < 0.05）。RARα表达可能是RA在体外对NB细胞产生作用所必需的，而该基因在体内表达的降低可能有助于NB肿瘤发生过程。
Zotero 插件