Shevell D E, Leu W M, Gillmor C S, Xia G, Feldmann K A, Chua N H
Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10021-6399.
Cell. 1994 Jul 1;77(7):1051-62. doi: 10.1016/0092-8674(94)90444-8.
The EMB30 gene is involved in apical-basal pattern formation in the Arabidopsis embryo. Mutations in this locus produce mutants with a wide range of seedling phenotypes, but all of the mutants lack a root and a true hypocotyl. We have cloned the EMB30 gene, and it encodes a protein that has similarity to the yeast Sec7 protein and to two other open reading frames identified in clones from humans and C. elegans. We refer to the region of similarity among these four sequences as the Sec7 domain. The emb30-1 allele has a mutation in the Sec7 domain that alters a residue conserved in all four of these sequences, suggesting that this domain may be important for EMB30 function. Molecular data and microscopy studies of emb30 seedlings presented here indicate that EMB30 affects cell division, elongation, and adhesion and functions in seedling and adult plants as well as during embryogenic pattern formation.
EMB30基因参与拟南芥胚胎的顶-基模式形成。该位点的突变产生具有多种幼苗表型的突变体，但所有突变体都缺乏根和真正的下胚轴。我们已经克隆了EMB30基因，它编码一种与酵母Sec7蛋白以及在人类和秀丽隐杆线虫克隆中鉴定出的另外两个开放阅读框相似的蛋白质。我们将这四个序列之间的相似区域称为Sec7结构域。emb30-1等位基因在Sec7结构域中有一个突变，该突变改变了所有这四个序列中保守的一个残基，这表明该结构域可能对EMB30的功能很重要。本文给出的emb30幼苗的分子数据和显微镜研究表明，EMB30影响细胞分裂、伸长和黏附，并且在幼苗和成株中以及胚胎模式形成过程中发挥作用。
